Liang Wanjun, Liu Shaopu, Song Jing, Hao Chenxia, Wang Linlin, Li Dan, He Youqiu
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
Biotechnol Lett. 2015 May;37(5):1057-61. doi: 10.1007/s10529-015-1767-7. Epub 2015 Jan 22.
A sensitive fluorescence biosensor for determining sparfloxacin (SPF) based on the electron transfer mechanism and the fluorescence quenching effect of SPF to cadmium telluride quantum dots (CdTe QDs) was developed. The mechanism of the interaction between SPF and CdTe QDs was investigated by UV/Vis absorption and fluorescence spectroscopy. The biosensor could be used for the determination of SPF with a high sensitivity. Under optimum conditions, the linear range was from 0.28 to 40 μg SPF ml(-1) with a correlation coefficient of 0.9983, and the detection limit (3δ/k) was 83.7 ng SPF ml(-1). Furthermore, this method has been applied to the determination of SPF in the synthetic environmental water samples and the spiked human serum samples with good results.
基于电子转移机制以及司帕沙星（SPF）对碲化镉量子点（CdTe QDs）的荧光猝灭效应，开发了一种用于测定司帕沙星（SPF）的灵敏荧光生物传感器。通过紫外/可见吸收光谱和荧光光谱研究了SPF与CdTe QDs之间的相互作用机制。该生物传感器可用于高灵敏度地测定SPF。在最佳条件下，线性范围为0.28至40 μg SPF ml(-1)，相关系数为0.9983，检测限（3δ/k）为83.7 ng SPF ml(-1)。此外，该方法已应用于合成环境水样和加标人血清样品中SPF的测定，结果良好。
