Berman S A, Bursztajn S, Kinnard R, Cashman N, Arnason B G
Department of Neurology, University of Chicago, Illinois 60637.
J Neurogenet. 1989 Nov;6(2):75-86. doi: 10.3109/01677068909107102.
The N-myc cellular oncogene is frequently amplified and expressed at a high level in neuroectodermal tumor cells such as neuroblastoma and retinoblastoma. We examined N-myc expression in NCB-20 hybrid (N18TG2 neuroblastoma x embryonic Chinese Hamster brain) cells. After five days of culture, cells treated with 1 mM db cAMP show extensive neurite outgrowth and secrete acetylcholinesterase into the media at a level three times higher than untreated control. In situ hybridizations, dot blots, and Northern analyses reveal four- to eight-fold higher levels of N-myc mRNA in the treated, differentiated cells than in the untreated, undifferentiated controls. Our results show that the highly differentiated state is not incompatible with a high level of N-myc mRNA.
N - myc细胞癌基因在神经外胚层肿瘤细胞（如神经母细胞瘤和视网膜母细胞瘤）中经常发生扩增并高水平表达。我们检测了NCB - 20杂交细胞（N18TG2神经母细胞瘤×中国仓鼠胚胎脑）中N - myc的表达。培养五天后，用1 mM二丁酰环磷腺苷（db cAMP）处理的细胞出现广泛的神经突生长，并向培养基中分泌乙酰胆碱酯酶，其水平比未处理的对照高两倍。原位杂交、斑点印迹和Northern分析显示，处理后的分化细胞中N - myc mRNA水平比未处理的未分化对照高4至8倍。我们的结果表明，高度分化状态与高水平的N - myc mRNA并非不相容。
