Cohrs R J, Torelli S, Prasad K N, Edwards-Prasad J, Sharma O K
Department of Molecular Biology, AMC Cancer Research Center, Denver, CO 80214.
Int J Dev Neurosci. 1991;9(2):187-94. doi: 10.1016/0736-5748(91)90011-a.
D-Alpha-tocopheryl succinate (vitamin E succinate) at a concentration of 11.3 microM inhibited growth and reduced the expression of c-myc, N-myc and H-ras specific mRNAs in murine neuroblastoma cells (NBP2) in culture. R020-1724 [4-(3-butoxy-4-methoxybenzyl)-2- imidazolidinone], an inhibitor of cyclic AMP phosphodiesterase, also inhibited growth and reduced the expression of these oncogenes. Vitamin E succinate treatment caused the formation of two c-myc related transcript of 1.9 and 3.7 kb; however, R020-1724 treatment did not. These results suggest that the inhibition of growth is sufficient to reduce the expression of c-myc, N-myc and H-ras in NB cells in culture, but it is not sufficient to produce two c-myc related transcripts.
浓度为11.3微摩尔的D-α-生育酚琥珀酸酯（维生素E琥珀酸酯）抑制了培养的小鼠神经母细胞瘤细胞（NBP2）的生长，并降低了c-myc、N-myc和H-ras特异性mRNA的表达。环磷酸腺苷磷酸二酯酶抑制剂R020-1724 [4-(3-丁氧基-4-甲氧基苄基)-2-咪唑啉酮] 也抑制了生长并降低了这些癌基因的表达。维生素E琥珀酸酯处理导致形成了两条大小分别为1.9和3.7 kb的与c-myc相关的转录本；然而，R020-1724处理并未导致这种情况。这些结果表明，生长抑制足以降低培养的神经母细胞瘤细胞中c-myc、N-myc和H-ras的表达，但不足以产生两条与c-myc相关的转录本。
