Nedvídková J, Stolba P
Research Institute of Endocrinology, Prague.
Physiol Bohemoslov. 1989;38(5):403-8.
The effect of the beta-adrenergic blockers L-alprenolol and DL-propranolol and of the beta-adrenergic agonist L-isoprenaline on the basal and thyrotropic hormone(TSH)-stimulated cyclic adenosine-monophosphate (cAMP) level in bovine thyroid slices was studied. The main basal cAMP level in bovine thyroid slices was 3 pmol/mg tissue. TSH stimulated cAMP production in correlation to the concentration. Maximum stimulation was achieved with a TSH concentration of 10 mU/ml. The beta-blockers DL-propranolol and L-alprenolol caused 74 and 77% inhibition of TSH-stimulated cAMP synthesis respectively. The beta-adrenergic agonist L-isoprenaline did not significantly affect either the basal or the TSH-stimulated cAMP level. The role of the beta-adrenergic receptor system in the regulation of TSH-stimulated cAMP synthesis is discussed.
研究了β-肾上腺素能阻滞剂L-阿普洛尔和DL-普萘洛尔以及β-肾上腺素能激动剂L-异丙肾上腺素对牛甲状腺切片基础及促甲状腺激素（TSH）刺激的环磷酸腺苷（cAMP）水平的影响。牛甲状腺切片中的主要基础cAMP水平为3 pmol/mg组织。TSH刺激cAMP产生，且与浓度相关。TSH浓度为10 mU/ml时达到最大刺激。β-阻滞剂DL-普萘洛尔和L-阿普洛尔分别导致TSH刺激的cAMP合成受到74%和77%的抑制。β-肾上腺素能激动剂L-异丙肾上腺素对基础或TSH刺激的cAMP水平均无显著影响。文中讨论了β-肾上腺素能受体系统在TSH刺激的cAMP合成调节中的作用。
