Kosik K S, Orecchio L D, Bakalis S, Neve R L
Department of Neurology (Neuroscience), Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Neuron. 1989 Apr;2(4):1389-97. doi: 10.1016/0896-6273(89)90077-9.
Tau protein undergoes a shift in its molecular mass and its electrophoretic complexity during early postnatal development. We have sequenced a tau cDNA from an adult rat brain expression library and have found two inserted sequences. One of these inserts predicts a fourth repeated sequence homologous to the other three in the carboxyl end of tau that have the property of microtubule binding. Oligonucleotide probes directed against the insert hybridized only to tau mRNA at postnatal time points, even though tau is first expressed as early as embryonic day 13. A probe directed against the junction revealed expression of non-insert-containing tau mRNA from embryonic day 14 until postnatal day 8, after which time there was an abrupt decline in the expression of this immature form. Comparison of the developmentally expressed tau sequences with those sequences obtained directly from Alzheimer paired helical filaments revealed the presence of both the mature and the immature tau mRNA sequences.
在出生后早期发育过程中，tau蛋白的分子量及其电泳复杂性会发生变化。我们从成年大鼠脑表达文库中对tau cDNA进行了测序，发现了两个插入序列。其中一个插入序列预测在tau蛋白羧基端有一个与其他三个重复序列同源的第四个重复序列，这三个重复序列具有微管结合特性。针对该插入序列的寡核苷酸探针仅在出生后的时间点与tau mRNA杂交，尽管tau最早在胚胎第13天就开始表达。针对连接处的探针显示，从胚胎第14天到出生后第8天，不含插入序列的tau mRNA有表达，此后这种未成熟形式的表达急剧下降。将发育过程中表达的tau序列与直接从阿尔茨海默病双螺旋丝获得的序列进行比较，发现同时存在成熟和未成熟的tau mRNA序列。
