Popov L S
Biokhimiia. 1989 Nov;54(11):1815-20.
The integration of temperature-sensitive SV40 mutant DNA (tsA239) into the Chinese hamster cellular genome at an early stage of infection was studied. The content of single-stranded DNA structures in the infected and control cells at a non-permissive temperature (40 degrees C) differed drastically from that in control cells at permissive temperatures (33 degrees C, 37 degrees C). The role of single-stranded structures in the integration of the SV40 genome into cellular DNA was shown by blot hybridization. The integration mechanism is discussed.
研究了温度敏感型SV40突变体DNA（tsA239）在感染早期整合到中国仓鼠细胞基因组中的情况。在非允许温度（40℃）下，感染细胞和对照细胞中双链DNA结构的含量与允许温度（33℃、37℃）下对照细胞中的含量有很大差异。通过印迹杂交显示了双链结构在SV40基因组整合到细胞DNA中的作用。讨论了整合机制。
