延伸因子（Elongator），一种真核生物中摆动尿苷修饰所需的保守复合物。
Elongator, a conserved complex required for wobble uridine modifications in eukaryotes.
作者信息
Karlsborn Tony, Tükenmez Hasan, Mahmud A K M Firoj, Xu Fu, Xu Hao, Byström Anders S
机构信息
a Department of Molecular Biology ; Umeå University; Umeå , Sweden.
出版信息
RNA Biol. 2014;11(12):1519-28. doi: 10.4161/15476286.2014.992276.
Abstract
Elongator is a 6 subunit protein complex highly conserved in eukaryotes. The role of this complex has been controversial as the pleiotropic phenotypes of Elongator mutants have implicated the complex in several cellular processes. However, in yeast there is convincing evidence that the primary and probably only role of this complex is in formation of the 5-methoxycarbonylmethyl (mcm(5)) and 5-carbamoylmethyl (ncm(5)) side chains on uridines at wobble position in tRNA. In this review we summarize the cellular processes that have been linked to the Elongator complex and discuss its role in tRNA modification and regulation of translation. We also describe additional gene products essential for formation of ncm(5) and mcm(5) side chains at U34 and their influence on Elongator activity.
摘要
延伸因子是一种在真核生物中高度保守的由6个亚基组成的蛋白质复合物。由于延伸因子突变体的多效性表型表明该复合物参与了多个细胞过程，其作用一直存在争议。然而，在酵母中有令人信服的证据表明，该复合物的主要（可能也是唯一）作用是在tRNA摆动位置的尿苷上形成5-甲氧羰基甲基（mcm(5)）和5-氨甲酰甲基（ncm(5)）侧链。在这篇综述中，我们总结了与延伸因子复合物相关的细胞过程，并讨论了其在tRNA修饰和翻译调控中的作用。我们还描述了在U34处形成ncm(5)和mcm(5)侧链所必需的其他基因产物及其对延伸因子活性的影响。
相似文献
1
Elongator, a conserved complex required for wobble uridine modifications in eukaryotes.
RNA Biol. 2014;11(12):1519-28. doi: 10.4161/15476286.2014.992276.
2
Elongator-a tRNA modifying complex that promotes efficient translational decoding.
Biochim Biophys Acta Gene Regul Mech. 2018 Apr;1861(4):401-408. doi: 10.1016/j.bbagrm.2017.11.006. Epub 2017 Nov 21.
3
Elongator, a conserved multitasking complex?
Mol Microbiol. 2010 Jun 1;76(5):1065-9. doi: 10.1111/j.1365-2958.2010.07162.x. Epub 2010 Apr 14.
4
Elongator function in tRNA wobble uridine modification is conserved between yeast and plants.
Mol Microbiol. 2010 Jun 1;76(5):1082-94. doi: 10.1111/j.1365-2958.2010.07163.x. Epub 2010 Apr 14.
5
A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex.
Mol Microbiol. 2014 Jun;92(6):1227-42. doi: 10.1111/mmi.12624. Epub 2014 May 19.
6
An early step in wobble uridine tRNA modification requires the Elongator complex.
RNA. 2005 Apr;11(4):424-36. doi: 10.1261/rna.7247705.
7
The role of wobble uridine modifications in +1 translational frameshifting in eukaryotes.
Nucleic Acids Res. 2015 Oct 30;43(19):9489-99. doi: 10.1093/nar/gkv832. Epub 2015 Aug 17.
8
SSD1 suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications.
PLoS Genet. 2019 Aug 29;15(8):e1008117. doi: 10.1371/journal.pgen.1008117. eCollection 2019 Aug.
9
Use of a Yeast tRNase Killer Toxin to Diagnose Kti12 Motifs Required for tRNA Modification by Elongator.
Toxins (Basel). 2017 Sep 5;9(9):272. doi: 10.3390/toxins9090272.
10
Eukaryotic life without tQCUG: the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum.
Nucleic Acids Res. 2020 Aug 20;48(14):7899-7913. doi: 10.1093/nar/gkaa560.
引用本文的文献
1
Effects of Nucleoside Modifications on mRNA Translation: Choosing the Right Modifications.
Methods Mol Biol. 2025;2965:127-149. doi: 10.1007/978-1-0716-4742-4_4.
2
Genetic modeling of ELP1-associated Sonic hedgehog medulloblastoma identifies MDM2 as a selective therapeutic target.
Cancer Cell. 2025 Jun 9;43(6):1141-1158.e11. doi: 10.1016/j.ccell.2025.04.014. Epub 2025 May 15.
3
mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery.
Nat Commun. 2025 May 6;16(1):4201. doi: 10.1038/s41467-025-59185-4.
4
mutants are temperature sensitive due to decay of tRNA by the 5'-3' exonuclease Dhp1, primarily targeting the unspliced pre-tRNA.
RNA. 2025 Mar 18;31(4):566-584. doi: 10.1261/rna.080315.124.
5
tRNA thiolation optimizes appressorium-mediated infection by enhancing codon-specific translation in Magnaporthe oryzae.
Nucleic Acids Res. 2025 Jan 7;53(1). doi: 10.1093/nar/gkae1302.
6
The Greatwall-Endosulfine-PP2A/B55 pathway regulates entry into quiescence by enhancing translation of Elongator-tunable transcripts.
Nat Commun. 2024 Dec 5;15(1):10603. doi: 10.1038/s41467-024-55004-4.
7
Investigating the role of RNA-binding protein Ssd1 in aneuploidy tolerance through network analysis.
RNA. 2024 Dec 16;31(1):100-112. doi: 10.1261/rna.080199.124.
8
Stress-Induced Eukaryotic Translational Regulatory Mechanisms.
J Clin Med Sci. 2024;8(2). Epub 2024 Jun 24.
9
Sod1-deficient cells are impaired in formation of the modified nucleosides mcmsU and yW in tRNA.
RNA. 2024 Nov 18;30(12):1586-1595. doi: 10.1261/rna.080181.124.
10
, the Gene Mutated in Familial Dysautonomia, Is Required for Normal Enteric Nervous System Development and Maintenance and for Gut Epithelium Homeostasis.
J Neurosci. 2024 Sep 11;44(37):e2253232024. doi: 10.1523/JNEUROSCI.2253-23.2024.
本文引用的文献
1
Familial dysautonomia (FD) patients have reduced levels of the modified wobble nucleoside mcm(5)s(2)U in tRNA.
Biochem Biophys Res Commun. 2014 Nov 21;454(3):441-5. doi: 10.1016/j.bbrc.2014.10.116. Epub 2014 Oct 30.
2
Archaeal Elp3 catalyzes tRNA wobble uridine modification at C5 via a radical mechanism.
Nat Chem Biol. 2014 Oct;10(10):810-2. doi: 10.1038/nchembio.1610. Epub 2014 Aug 24.
3
The cytosolic thiouridylase CTU2 of Arabidopsis thaliana is essential for posttranscriptional thiolation of tRNAs and influences root development.
BMC Plant Biol. 2014 Apr 28;14:109. doi: 10.1186/1471-2229-14-109.
4
A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex.
Mol Microbiol. 2014 Jun;92(6):1227-42. doi: 10.1111/mmi.12624. Epub 2014 May 19.
5
Role of tRNA modifications in human diseases.
Trends Mol Med. 2014 Jun;20(6):306-14. doi: 10.1016/j.molmed.2014.01.008. Epub 2014 Feb 25.
6
Ribosome profiling: new views of translation, from single codons to genome scale.
Nat Rev Genet. 2014 Mar;15(3):205-13. doi: 10.1038/nrg3645. Epub 2014 Jan 28.
7
Dph3 is an electron donor for Dph1-Dph2 in the first step of eukaryotic diphthamide biosynthesis.
J Am Chem Soc. 2014 Feb 5;136(5):1754-7. doi: 10.1021/ja4118957. Epub 2014 Jan 22.
8
Loss of a conserved tRNA anticodon modification perturbs cellular signaling.
PLoS Genet. 2013;9(8):e1003675. doi: 10.1371/journal.pgen.1003675. Epub 2013 Aug 1.
9
Modification of tRNA(Lys) UUU by elongator is essential for efficient translation of stress mRNAs.
PLoS Genet. 2013;9(7):e1003647. doi: 10.1371/journal.pgen.1003647. Epub 2013 Jul 18.
10
tRNA tKUUU, tQUUG, and tEUUC wobble position modifications fine-tune protein translation by promoting ribosome A-site binding.
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12289-94. doi: 10.1073/pnas.1300781110. Epub 2013 Jul 8.
Zotero 插件