表面增强振动光谱证据表明内切酶 III 的一种替代的非 DNA 依赖性氧化还原激活。

Surface enhanced vibrational spectroscopic evidence for an alternative DNA-independent redox activation of endonuclease III.

机构信息

Instituto de Tecnologia Quimica e Biologica (ITQB), Universidade Nova de Lisboa, Av. da Republica (EAN), 2780-157 Oeiras, Portugal.

出版信息

Chem Commun (Camb). 2015 Feb 21;51(15):3255-7. doi: 10.1039/c4cc09498k.

DOI:10.1039/c4cc09498k

PMID:25608720

Abstract

Surface enhanced vibrational spectro-electrochemistry of endonuclease III provides direct evidence that the [4Fe-4S] cluster is responsible for the enzyme redox activity, and that this process is not exclusively DNA-mediated, as currently proposed. We report the first surface enhanced resonance Raman spectrum of a 4Fe-4S cluster containing enzyme.

摘要

内切酶 III 的表面增强振动光谱电化学提供了直接证据，证明 [4Fe-4S] 簇负责酶的氧化还原活性，而且这个过程并不像目前所提出的那样完全是由 DNA 介导的。我们报告了首例含有 4Fe-4S 簇的酶的表面增强共振拉曼光谱。