Cao C F
Zhonghua Kou Qiang Yi Xue Za Zhi. 1989 Jul;24(4):204-7, 254.
The volume and myeloperoxidase (MPO) activity of gingival crevicular fluid (GCF) collected with filter paper strips for 30 sec from the sulcus of healthy, gingivitis and periodontitis sites of Chinese subjects were measured. MPO/site and MPO/microliter GCF were both greater at gingivitis and periodontitis sites than healthy sites. Enzyme activity was similar at the 2 categories of diseased sites. Mean GCF volume and MPO activity of the samples were calculated for all sites with GI 0, 1, 2 and 0 + 1 irrespective of experimental group assignment. GCF volume MPO/site and MPO/microliter GCF all were greater at GI 2 than GI 0 or 0 + 1. These data indicate that increased GCF MPO previously observed at periodontitis sites is not specific to these sites. Rather, increased GCF MPO likely occurs when additional polymorphonuclear leukocytes enter the sulcus as a result of gingival inflammation. A second sample was obtained from 22 sites 4 weeks after the initial collection. These samples were collected for 5 rather than 30 sec. The GCF volume, MPO/site and MPO/microliter GCF each were greater in samples collected for 30 rather than 5 sec. Correlation coefficients showed that the amount of GCF and MPO activity of the fluid collected for 5 and 30 sec was dependent upon the site even though the samples were collected at different times.
采用滤纸条从中国受试者健康、牙龈炎和牙周炎部位的龈沟采集龈沟液（GCF）30秒，测量其体积和髓过氧化物酶（MPO）活性。在牙龈炎和牙周炎部位，MPO/部位和MPO/微升GCF均高于健康部位。在两类患病部位，酶活性相似。计算所有GI为0、1、2以及0 + 1的部位样本的平均GCF体积和MPO活性，不考虑实验组分配情况。GI为2时，GCF体积、MPO/部位和MPO/微升GCF均高于GI为0或0 + 1时。这些数据表明，先前在牙周炎部位观察到的GCF MPO增加并非这些部位所特有。相反，当牙龈炎症导致更多多形核白细胞进入龈沟时，GCF MPO可能会增加。在首次采集后4周，从22个部位获取了第二个样本。这些样本采集5秒而非30秒。采集30秒的样本中，GCF体积、MPO/部位和MPO/微升GCF均大于采集5秒的样本。相关系数表明，尽管样本在不同时间采集，但采集5秒和30秒的液体中GCF量和MPO活性均取决于部位。
