Mengaud J, Vicente M F, Chenevert J, Geoffroy C, Baquero F, Perez-Diaz J C, Cossart P
Unit of Microbial Engineering, Institute Pasteur, Paris, France.
Acta Microbiol Hung. 1989;36(2-3):177-82.
The locus of insertion of a transposon previously used to obtain a non-haemolytic avirulent mutant was identified: it is the structural gene encoding lisreriolysin O, the thiol-dependent haemolysin, now called hlyA. The gene was completely sequenced. The preliminary structural and functional study of the chromosomal region containing the gene indicates that hlyA belongs to a monocistronic transcriptional unit. If it is the case, the transposon insertion would have no major polar effect on downstream genes and would only affect hlyA expression. These results emphasize the importance of the haemolysin in the virulence of Listeria monocytogenes.
它是编码李斯特菌溶血素O的结构基因，李斯特菌溶血素O是一种硫醇依赖性溶血素，现称为hlyA。该基因已被完全测序。对包含该基因的染色体区域进行的初步结构和功能研究表明，hlyA属于一个单顺反子转录单元。如果是这种情况，转座子插入对下游基因不会产生重大的极性效应，只会影响hlyA的表达。这些结果强调了溶血素在单核细胞增生李斯特菌毒力中的重要性。
