Cossart P
Département des Biotechnologies, Institut Pasteur, Paris.
Infection. 1988;16 Suppl 2:S157-9. doi: 10.1007/BF01639740.
In culture supernatants of a Tn 1545-induced non-hemolytic mutant of Listeria monocytogenes, by immunoblotting with an anti-serum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52,000D (the secreted listeriolysin O is 60,000D). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame. The homologies detected between this ORF, streptolysin O and pneumolysin demonstrate the the transposon had indeed inserted in the listeriolysin O gene. As the non-hemolytic mutant was non-virulent, our work demonstrated that a genetic determinant essential for virulence is the listeriolysin O gene or its adjacent region.
在单核细胞增生李斯特菌Tn1545诱导的非溶血性突变体的培养上清液中，通过用针对纯化的李斯特菌溶血素O产生的抗血清进行免疫印迹，我们检测到存在一种52,000D的截短蛋白（分泌的李斯特菌溶血素O为60,000D）。转座子的插入区域已被克隆和测序。转座子插入了一个开放阅读框。在这个开放阅读框、链球菌溶血素O和肺炎球菌溶血素之间检测到的同源性表明转座子确实插入了李斯特菌溶血素O基因。由于非溶血性突变体无毒力，我们的工作表明毒力所必需的遗传决定因素是李斯特菌溶血素O基因或其相邻区域。
