Mengaud J, Chenevert J, Geoffroy C, Gaillard J L, Cossart P
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Infect Immun. 1987 Dec;55(12):3225-7. doi: 10.1128/iai.55.12.3225-3227.1987.
By immunoblotting with an antiserum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52 kilodaltons in culture supernatants of a Tn1545-induced nonhemolytic mutant of Listeria monocytogenes (J.L. Gaillard, P. Berche, and P. Sansonetti, Infect. Immun. 52:50-55, 1986). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame the listeriolysin O gene. The deduced amino acid sequence of this open reading frame revealed that listeriolysin O is homologous to streptolysin O and pneumolysin, although homologies were not detectable at the DNA level.
通过用针对纯化的李斯特菌溶血素O制备的抗血清进行免疫印迹，我们在单核细胞增生李斯特菌的Tn1545诱导的非溶血突变体（J.L. 盖拉德、P. 贝什和P. 桑索尼蒂，《感染与免疫》52:50 - 55，1986年）的培养上清液中检测到了一种52千道尔顿的截短蛋白。转座子的插入区域已被克隆并测序。转座子插入了李斯特菌溶血素O基因的一个开放阅读框中。该开放阅读框推导的氨基酸序列显示，李斯特菌溶血素O与链球菌溶血素O和肺炎球菌溶血素同源，尽管在DNA水平上未检测到同源性。
