School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China ; School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
Evid Based Complement Alternat Med. 2014;2014:605914. doi: 10.1155/2014/605914. Epub 2014 Dec 25.
Most research focuses on the hypothalamic-pituitary-adrenal (HPA) axis, hypothalamus-pituitary-thyroid (HPT) axis, and hypothalamus-pituitary-gonadal (HPGA) axis systems of abnormalities of emotions and behaviors induced by stress, while no studies of Chinese herbal medicine such as Xiao Yao San (XYS) on the mechanisms of locus coeruleus-norepinephrine (LC-NE) system have been reported. Therefore, experiments were carried out to observe mechanism of LC-NE system in response to chronic immobilization stress (CIS) and explore the antidepressant effect of XYS. Rat model was established by CIS. LC morphology in rat was conducted. The serum norepinephrine (NE) concentrations and NE biosynthesis such as tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and corticotrophin-releasing-factor (CRF) in LC were determined. Results showed that there were no discernible alterations in LC in rats. The serum NE concentrations, positive neurons, mean optical density (MOD), and protein levels of TH, DBH, and CRF in model group were significantly increased compared to the control group. But XYS-treated group displayed a significantly decreased in NE levels and expressions of TH, DBH, and CRF compared to the model group. In conclusion, CIS can activate LC-NE system to release NE and then result in a significant decrease in rats. XYS treatment can effectively improve depressive-like behaviors in rats through inhibition of LC-NE neurons activity.
大多数研究都集中在应激引起的情绪和行为异常的下丘脑-垂体-肾上腺（HPA）轴、下丘脑-垂体-甲状腺（HPT）轴和下丘脑-垂体-性腺（HPGA）轴系统上，而没有关于中药如逍遥散（XYS）对蓝斑-去甲肾上腺素（LC-NE）系统机制的研究。因此，进行了实验以观察 LC-NE 系统对慢性束缚应激（CIS）的反应机制，并探讨 XYS 的抗抑郁作用。通过 CIS 建立大鼠模型。对大鼠 LC 形态进行了研究。测定 LC 中血清去甲肾上腺素（NE）浓度和 NE 生物合成酶如酪氨酸羟化酶（TH）、多巴胺-β-羟化酶（DBH）和促肾上腺皮质释放因子（CRF）。结果表明，大鼠 LC 无明显改变。与对照组相比，模型组血清 NE 浓度、阳性神经元、平均光密度（MOD）以及 TH、DBH 和 CRF 的蛋白水平显著升高。但与模型组相比，XYS 治疗组 NE 水平和 TH、DBH 和 CRF 的表达明显降低。综上所述，CIS 可激活 LC-NE 系统释放 NE，导致大鼠 NE 显著减少。XYS 治疗可通过抑制 LC-NE 神经元活性有效改善大鼠的抑郁样行为。
