Houff S A, Katz D, Kufta C V, Major E O
Section on Molecular Virology and Genetics, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
AIDS. 1989 Dec;3(12):843-5. doi: 10.1097/00002030-198912000-00010.
Brain biopsy is often necessary in the diagnosis of neurological complications found in AIDS patients. We describe here a rapid method of tissue preparation and in situ DNA hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the diagnosis of progressive multifocal leukoencephalopathy to be established on the day of surgery. Once the diagnosis is established, therapeutic and management decisions can be made more easily. The commercial availability of biotinylated probes for several of the DNA viruses most frequently encountered in brain infections of AIDS patients will provide wide application of these techniques to patient management.
脑活检对于诊断艾滋病患者的神经并发症常常是必要的。我们在此描述一种快速的组织制备和原位DNA杂交方法，用于在冷冻脑活检切片中检测JC病毒DNA，这使得在手术当天就能确诊进行性多灶性白质脑病。一旦确诊，就能更容易地做出治疗和管理决策。用于艾滋病患者脑部感染中最常遇到的几种DNA病毒的生物素化探针的商业可得性，将使这些技术在患者管理中得到广泛应用。
