• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

骨髓细胞亚群沿SDF-1α和ATP梯度的选择性迁移。

Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient.

作者信息

Laupheimer Michael, Skorska Anna, Große Jana, Tiedemann Gudrun, Steinhoff Gustav, David Robert, Lux Cornelia A

机构信息

Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock, 18057 Rostock, Germany.

Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock, 18057 Rostock, Germany ; RTC, BMFZ, Schillingallee 68, 18057 Rostock, Germany.

出版信息

Bone Marrow Res. 2014;2014:182645. doi: 10.1155/2014/182645. Epub 2014 Dec 31.

DOI:10.1155/2014/182645
PMID:25610653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4294463/
Abstract

Both stem cell chemokine stromal cell-derived factor-1α (SDF-1α) and extracellular nucleotides such as adenosine triphosphate (ATP) are increased in ischemic myocardium. Since ATP has been reported to influence cell migration, we analysed the migratory response of bone marrow cells towards a combination of SDF-1 and ATP. Total nucleated cells (BM-TNCs) were isolated from bone marrow of cardiac surgery patients. Migration assays were performed in vitro. Subsequently, migrated cells were subjected to multicolor flow cytometric analysis of CD133, CD34, CD117, CD184, CD309, and CD14 expression. BM-TNCs migrated significantly towards a combination of SDF-1 and ATP. The proportions of CD34+ cells as well as subpopulations coexpressing multiple stem cell markers were selectively enhanced after migration towards SDF-1 or SDF-1 + ATP. After spontaneous migration, significantly fewer stem cells and CD184+ cells were detected. Direct incubation with SDF-1 led to a reduction of CD184+ but not stem cell marker-positive cells, while incubation with ATP significantly increased CD14+ percentage. In summary, we found that while a combination of SDF-1 and ATP elicited strong migration of BM-TNCs in vitro, only SDF-1 was responsible for selective attraction of hematopoietic stem cells. Meanwhile, spontaneous migration of stem cells was lower compared to BM-TNCs or monocytes.

摘要

干细胞趋化因子基质细胞衍生因子-1α（SDF-1α）和细胞外核苷酸如三磷酸腺苷（ATP）在缺血心肌中均会增加。由于已有报道称ATP会影响细胞迁移，我们分析了骨髓细胞对SDF-1和ATP组合的迁移反应。从心脏手术患者的骨髓中分离出有核细胞总数（BM-TNCs）。在体外进行迁移试验。随后，对迁移的细胞进行CD133、CD34、CD117、CD184、CD309和CD14表达的多色流式细胞术分析。BM-TNCs对SDF-1和ATP的组合有显著迁移。向SDF-1或SDF-1 + ATP迁移后，CD34+细胞以及共表达多种干细胞标志物的亚群比例选择性增加。自发迁移后，检测到的干细胞和CD184+细胞明显减少。与SDF-1直接孵育导致CD184+细胞减少，但干细胞标志物阳性细胞未减少，而与ATP孵育显著增加了CD14+百分比。总之，我们发现虽然SDF-1和ATP的组合在体外引起BM-TNCs的强烈迁移，但只有SDF-1负责造血干细胞的选择性吸引。同时，与BM-TNCs或单核细胞相比，干细胞的自发迁移较低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/ad19ecc9086d/BMR2014-182645.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/8ee15e6cb844/BMR2014-182645.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/b0fc562291ce/BMR2014-182645.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/3efcfebe9d2c/BMR2014-182645.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/f0c57c190030/BMR2014-182645.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/d7a08ffc7518/BMR2014-182645.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/ad19ecc9086d/BMR2014-182645.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/8ee15e6cb844/BMR2014-182645.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/b0fc562291ce/BMR2014-182645.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/3efcfebe9d2c/BMR2014-182645.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/f0c57c190030/BMR2014-182645.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/d7a08ffc7518/BMR2014-182645.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/4294463/ad19ecc9086d/BMR2014-182645.006.jpg

相似文献

1
Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient.骨髓细胞亚群沿SDF-1α和ATP梯度的选择性迁移。
Bone Marrow Res. 2014;2014:182645. doi: 10.1155/2014/182645. Epub 2014 Dec 31.
2
Increased migration of cord blood-derived CD34+ cells, as compared to bone marrow and mobilized peripheral blood CD34+ cells across uncoated or fibronectin-coated filters.与骨髓和动员外周血中的CD34+细胞相比，脐血来源的CD34+细胞在未包被或纤连蛋白包被的滤膜上的迁移增加。
Exp Hematol. 1999 Dec;27(12):1806-14. doi: 10.1016/s0301-472x(99)00113-7.
3
Chemokine stromal cell-derived factor-1alpha modulates VLA-4 integrin-dependent adhesion to fibronectin and VCAM-1 on bone marrow hematopoietic progenitor cells.趋化因子基质细胞衍生因子-1α调节骨髓造血祖细胞上VLA-4整合素依赖的对纤连蛋白和血管细胞黏附分子-1的黏附。
Exp Hematol. 2001 Mar;29(3):345-55. doi: 10.1016/s0301-472x(00)00668-8.
4
Progenitor egress from the bone marrow after allergen challenge: role of stromal cell-derived factor 1alpha and eotaxin.变应原激发后祖细胞从骨髓中迁出：基质细胞衍生因子1α和嗜酸性粒细胞趋化因子的作用
J Allergy Clin Immunol. 2005 Mar;115(3):501-7. doi: 10.1016/j.jaci.2004.11.017.
5
Migratory behavior of leukemic cells from acute myeloid leukemia patients.急性髓系白血病患者白血病细胞的迁移行为。
Leukemia. 2002 Apr;16(4):650-7. doi: 10.1038/sj.leu.2402431.
6
Analyzing migratory properties of human CD133(+) stem cells in vivo after intraoperative sternal bone marrow isolation.分析术中胸骨骨髓分离后体内人 CD133(+)干细胞的迁移特性。
Cell Transplant. 2013;22(9):1627-35. doi: 10.3727/096368912X657729. Epub 2012 Oct 8.
7
Biological effects of stroma-derived factor-1 alpha on normal and CML CD34+ haemopoietic cells.基质衍生因子-1α对正常和慢性粒细胞白血病CD34+造血细胞的生物学效应。
Leukemia. 2000 Sep;14(9):1652-60. doi: 10.1038/sj.leu.2401875.
8
Treatment of circulating CD34(+) cells with SDF-1alpha or anti-CXCR4 antibody enhances migration and NOD/SCID repopulating potential.用SDF-1α或抗CXCR4抗体处理循环CD34(+)细胞可增强其迁移能力和在非肥胖型糖尿病/重症联合免疫缺陷小鼠中的重建潜力。
Exp Hematol. 2002 Sep;30(9):1061-9. doi: 10.1016/s0301-472x(02)00880-9.
9
Endothelial nitric oxide synthase promotes bone marrow stromal cell migration to the ischemic myocardium via upregulation of stromal cell-derived factor-1alpha.内皮型一氧化氮合酶通过上调基质细胞衍生因子-1α促进骨髓基质细胞向缺血心肌迁移。
Stem Cells. 2009 Apr;27(4):961-70. doi: 10.1002/stem.6.
10
The neurotransmitter GABA is a potent inhibitor of the stromal cell-derived factor-1alpha induced migration of adult CD133+ hematopoietic stem and progenitor cells.神经递质γ-氨基丁酸（GABA）是基质细胞衍生因子-1α诱导的成年CD133+造血干细胞和祖细胞迁移的强效抑制剂。
Stem Cells Dev. 2007 Oct;16(5):827-36. doi: 10.1089/scd.2007.0004.

引用本文的文献

1
Mechanisms of stem cell based cardiac repair-gap junctional signaling promotes the cardiac lineage specification of mesenchymal stem cells.基于干细胞的心脏修复机制-缝隙连接信号促进间充质干细胞向心脏谱系的特化。
Sci Rep. 2017 Aug 29;7(1):9755. doi: 10.1038/s41598-017-10122-6.
2
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy.利用三维荧光漂白恢复显微镜分析微小RNA的间隙连接依赖性转移
J Vis Exp. 2017 Jun 19(124):55870. doi: 10.3791/55870.
3
GMP-conformant on-site manufacturing of a CD133 stem cell product for cardiovascular regeneration.

本文引用的文献

1
Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells.核纤层蛋白调节成人人类造血细胞的细胞运输和谱系成熟。
Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):18892-7. doi: 10.1073/pnas.1304996110. Epub 2013 Nov 4.
2
ATP release and purinergic signaling: a common pathway for particle-mediated inflammasome activation.三磷酸腺苷释放和嘌呤能信号转导：颗粒介导的炎症小体激活的共同途径。
Cell Death Dis. 2012 Oct 11;3(10):e403. doi: 10.1038/cddis.2012.144.
3
The expanding family of bone marrow homing factors for hematopoietic stem cells: stromal derived factor 1 is not the only player in the game.
用于心血管再生的CD133干细胞产品的符合GMP规范的现场生产。
Stem Cell Res Ther. 2017 Feb 10;8(1):33. doi: 10.1186/s13287-016-0467-0.
造血干细胞骨髓归巢因子的扩展家族：基质衍生因子1并非唯一参与者。
ScientificWorldJournal. 2012;2012:758512. doi: 10.1100/2012/758512. Epub 2012 Jun 4.
4
New insights regarding the regulation of chemotaxis by nucleotides, adenosine, and their receptors.核苷酸、腺苷及其受体调节趋化作用的新见解。
Purinergic Signal. 2012 Sep;8(3):587-98. doi: 10.1007/s11302-012-9311-x. Epub 2012 Apr 15.
5
Cardiac stem cell therapy. Registered trials and a pilot study in patients with dilated cardiomyopathy.心脏干细胞疗法。扩张型心肌病患者的注册试验及一项试点研究。
Herz. 2011 Mar;36(2):121-34. doi: 10.1007/s00059-010-3419-y.
6
Intramyocardial bone marrow stem cell transplantation during coronary artery bypass surgery: a meta-analysis.冠状动脉旁路移植术中心肌内骨髓干细胞移植：荟萃分析。
J Thorac Cardiovasc Surg. 2011 Oct;142(4):911-20. doi: 10.1016/j.jtcvs.2010.12.013. Epub 2011 Mar 3.
7
Hematopoietic stem cell homing to injured tissues.造血干细胞归巢至损伤组织。
Stem Cell Rev Rep. 2011 Sep;7(3):672-82. doi: 10.1007/s12015-011-9240-z.
8
Bone marrow resident and circulating progenitor cells in patients undergoing cardiac surgery.心脏手术患者骨髓固有和循环祖细胞。
Ann Thorac Surg. 2010 Dec;90(6):1944-51. doi: 10.1016/j.athoracsur.2010.08.033.
9
High CD4/CD8 ratio in allografts predicts adverse outcomes in unmanipulated HLA-mismatched/haploidentical hematopoietic stem cell transplantation for chronic myeloid leukemia.在慢性髓性白血病的非处理 HLA 不匹配/单倍体相合造血干细胞移植中，移植物中高 CD4/CD8 比值预示不良预后。
Ann Hematol. 2009 Oct;88(10):1015-24. doi: 10.1007/s00277-009-0728-4. Epub 2009 Mar 25.
10
Intracoronary administration of bone marrow-derived progenitor cells improves left ventricular function in patients at risk for adverse remodeling after acute ST-segment elevation myocardial infarction: results of the Reinfusion of Enriched Progenitor cells And Infarct Remodeling in Acute Myocardial Infarction study (REPAIR-AMI) cardiac magnetic resonance imaging substudy.急性ST段抬高型心肌梗死后有不良重塑风险的患者，冠状动脉内注射骨髓源性祖细胞可改善左心室功能：急性心肌梗死中富集祖细胞再输注与梗死重塑研究（REPAIR-AMI）心脏磁共振成像子研究结果
Am Heart J. 2009 Mar;157(3):541-7. doi: 10.1016/j.ahj.2008.11.011. Epub 2009 Jan 31.