Wan Hong-Ye, Kong Xiang-Ying, Li Xiao-Min, Zhu Hong-Wei, Su Xiao-Hui, Lin Na
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(19):3813-8.
To compare the intervention effects of four traditional Chinese medicines (TCMs) with typical cold or hot property on body temperature and temperature-sensitive transient receptor potential ion channel proteins (TRPs) of rats with yeast-induced fever.
The pyrexia model was induced by injecting yeast suspension subcutaneously. Totally 108 male SD rats were randomly divided into the normal group, the model group, the Rhei Radix et Rhizoma treated group, the Coptidis Rhizoma treated group, the Euodiae Fructus treated group, and the Alpiniae Officinarum Rhizoma treated group, with 18 rats in each group. At the 4 h, 8 h and 12 h after injection of yeast, the rats were sacrificed to collect their hypothalamus and dorsal root ganglion. The expressions of TRPV1 and TRPM8 were detected by immunohistochemistry and Western blot method.
Compared with the normal group, after injection of yeast, the temperature of rats in the model group notably increased, and reached the peak at 8 h (P < 0.01). The TRPV1 level in hypothalamus and dorsal root ganglia (DRG) of the model group significantly increased, whereas the TRPM8 level significantly reduced. Compared with the model group, the Rhei Radix et Rhizoma group and the Coptidis Rhizoma group showed significant decrease in the high body temperature of rats caused by yeast, down-regulation in the expression of TRPV1, and up-regulation in the expression of TRPM8 (P < 0.05 or P < 0.01). Euodiae Fructus and Alpiniae Officinarum Rhizoma had no significant effect on either temperature or TRPs of fever rats.
Rhei Radix et Rhizoma and Coptidis Rhizoma, both are TCMs with cold property, can reduce the temperature of fever rats induced by yeast, which may be related to their effective regulation of TRPV1 and TRPM8 in hypothalamus and DRG, while Euodiae Fructus and Alpiniae Officinarum Rhizoma had no relevant effect.
比较四种典型寒、热属性的中药对酵母致热大鼠体温及温度敏感型瞬时受体电位离子通道蛋白（TRPs）的干预作用。
皮下注射酵母悬液制备发热模型。将108只雄性SD大鼠随机分为正常组、模型组、大黄治疗组、黄连治疗组、吴茱萸治疗组和高良姜治疗组，每组18只。于注射酵母后4 h、8 h和12 h处死大鼠，取下丘脑和背根神经节。采用免疫组织化学和蛋白质印迹法检测TRPV1和TRPM8的表达。
与正常组比较，注射酵母后模型组大鼠体温显著升高，8 h达峰值（P<0.01）。模型组下丘脑和背根神经节（DRG）中TRPV1水平显著升高，而TRPM8水平显著降低。与模型组比较，大黄组和黄连组大鼠由酵母引起的高热显著降低，TRPV1表达下调，TRPM8表达上调（P<0.05或P<0.01）。吴茱萸和高良姜对发热大鼠的体温和TRPs均无显著影响。
寒性中药大黄和黄连可降低酵母致热大鼠的体温，可能与其有效调节下丘脑和DRG中的TRPV1和TRPM8有关，而吴茱萸和高良姜无此作用。
