Konečná B, Borbélyová V, Celec P, Vlková B
1Institute of Molecular Biomedicine, Faculty of Medicine,Comenius University,Bratislava,Slovakia.
J Dev Orig Health Dis. 2015 Feb;6(1):5-9. doi: 10.1017/S2040174414000622.
Cell-free fetal DNA in maternal circulation is higher during preeclampsia. It is unclear whether it is the cause or the consequence of the disease. The aim of this study was to prove whether injected rat fetal DNA induces preeclampsia-like symptoms in pregnant Wistar rats. They received daily i.p. injections of water or rat fetal DNA (400 μg) from gestation day 14 to 18. Blood pressure, proteinuria, placental and fetal weight were measured at gestation day 19. Plasma DNase activity, proteinuria and creatinine clearance were assessed. There was no significant difference in any of the measured parameters. The results of this study do not confirm the hypothesis that fetal DNA might induce preeclampsia. This is in contrast to others using human fetal DNA in mice. Further studies should be focused on the effects of fetal DNA from the same species protected from DNase activity.
子痫前期孕妇循环中的游离胎儿DNA水平较高。目前尚不清楚这是该疾病的原因还是结果。本研究的目的是证明注射大鼠胎儿DNA是否会在怀孕的Wistar大鼠中诱发子痫前期样症状。从妊娠第14天至18天，它们每天接受腹腔注射水或大鼠胎儿DNA（400μg）。在妊娠第19天测量血压、蛋白尿、胎盘和胎儿重量。评估血浆DNase活性、蛋白尿和肌酐清除率。在所测量的任何参数中均无显著差异。本研究结果未证实胎儿DNA可能诱发子痫前期的假设。这与其他在小鼠中使用人类胎儿DNA的研究结果相反。进一步的研究应集中于免受DNase活性影响的同一物种胎儿DNA的作用。
