Efimtsev E I, Burov G P, Solomin A A, Iudina T G, Egorov N S
Antibiot Khimioter. 1989 Nov;34(11):841-6.
New methods were developed and applied to quantitative determination of beta-exotoxin and antibiotic activity of delta-endotoxin with respect to Micrococcus spp. in bitoxibacillin (BTB) and the fermentation broths prepared under industrial conditions. The biosynthesis of beta-exotoxin in the period of its maximum accumulation during the fermentation was estimated. It was shown that the primary biological effect of BTB on insects consisted in the actions of beta-exotoxin and delta-endotoxin. Biological activity of each of the entomocidal components of the entomocidal components of BTB did not practically correlated with the number of viable spores. There was a correlation between the antibiotic activity of crystalline B. thuringiensis subsp. thuringiensis solutions and the insecticidal activity of the entomopathogenic preparations. Determination of beta-exotoxin and antibiotic activity of delta-endotoxin might be used as a complex procedure for testing the quality of BTB. The method for estimating antibiotic activity of the crystal solutions allowed one to assay the biological activity of other preparations based on Bacillus thuringiensis non-synthesizing beta-exotoxin.
开发了新方法并应用于定量测定比托西菌素（BTB）及工业条件下制备的发酵液中β-外毒素的含量以及δ-内毒素对微球菌属的抗菌活性。评估了发酵过程中β-外毒素在其最大积累期的生物合成情况。结果表明，BTB对昆虫的主要生物学作用在于β-外毒素和δ-内毒素的作用。BTB杀虫成分中每种杀虫成分的生物活性实际上与活孢子数量无关。苏云金芽孢杆菌亚种苏云金芽孢杆菌结晶溶液的抗菌活性与昆虫病原体制剂的杀虫活性之间存在相关性。测定β-外毒素和δ-内毒素的抗菌活性可作为检测BTB质量的综合程序。晶体溶液抗菌活性的评估方法可用于测定其他基于不合成β-外毒素的苏云金芽孢杆菌的制剂的生物活性。
