Abrosimova L I, Leskova A Ia, Babaeva P V, Shevtsov V V
Mikrobiologiia. 1985 Sep-Oct;54(5):770-3.
Various procedures were studied for obtaining Bacillus thuringiensis strains of serotype I which synthesized exotoxins. Mutant clones with elevated exotoxin synthesis could be selected by treating the cells with N-methyl-N-nitro-N-nitrosoguanidine. The frequency of (+) variant selection was from 17 to 12 X 10(-2). The clones of S and R types differed in the insecticide activity of the exotoxin. Its yield could be increased by optimizing the composition of growth media. The strain specificity of B. thuringiensis producing the exotoxin was assayed in terms of carbon and nitrogen requirements.
对获取合成外毒素的I型苏云金芽孢杆菌菌株的各种程序进行了研究。通过用N-甲基-N-硝基-N-亚硝基胍处理细胞，可以选择外毒素合成增加的突变克隆。（+）变体选择的频率为17至12×10⁻²。S型和R型克隆在外毒素的杀虫活性方面存在差异。通过优化生长培养基的组成可以提高其产量。根据碳和氮需求测定了产生外毒素的苏云金芽孢杆菌的菌株特异性。
