Wang Jing-Rong, Guan Wen-Da, Yau Lee-Fong, Gao Wei-Na, Zhan Yang-Qing, Liu Liang, Yang Zi-Feng, Jiang Zhi-Hong
State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Sci Rep. 2015 Jan 23;5:7648. doi: 10.1038/srep07648.
Millions of individuals are vaccinated worldwide each year to stimulate their adaptive immune systems to produce protective antibodies and T-cell response against pathogens. Since glycosylation of the Fc region of immunoglobulin G (IgG) can be influenced by the host's immune status, it was inferred that glycosylation profile of IgG might be altered as a result of the immune response. Therefore, subclass-specific glycosylation profiles of serum IgGs from 26 healthy adults before and after vaccination with a trivalent subunit influenza virus vaccine were comprehensively analyzed to explore glycomic signatures for vaccination. The results showed that no significant changes in the glycosylation of total IgGs took place before and after vaccination, but distinct glycosylation profiles in responders (fourfold or more increase of HI titer after vaccination) and nonresponders (less than fourfold increase of HI titer) were observed. This difference between the responders and nonresponders occurred even in the resting state. On the basis of variable importance parameters, glycosylation markers that distinguish responders from nonresponders were identified. These markers can be used as molecular signatures to predict antibody titers after vaccination. This is the first study of serum IgG glycosylation profiles in healthy adults receiving a trivalent inactivated influenza vaccine.
每年全球有数百万人接种疫苗，以刺激其适应性免疫系统产生针对病原体的保护性抗体和T细胞反应。由于免疫球蛋白G（IgG）Fc区的糖基化会受到宿主免疫状态的影响，因此推测IgG的糖基化谱可能会因免疫反应而改变。因此，全面分析了26名健康成年人在接种三价亚单位流感病毒疫苗前后血清IgG的亚类特异性糖基化谱，以探索疫苗接种的糖组学特征。结果显示，接种疫苗前后总IgG的糖基化没有显著变化，但在应答者（接种疫苗后HI滴度增加四倍或更多）和无应答者（HI滴度增加少于四倍）中观察到了不同的糖基化谱。即使在静息状态下，应答者和无应答者之间也存在这种差异。基于变量重要性参数，确定了区分应答者和无应答者的糖基化标志物。这些标志物可作为分子特征来预测接种疫苗后的抗体滴度。这是首次对接受三价灭活流感疫苗的健康成年人血清IgG糖基化谱进行的研究。
