Ramar Manikandan, Manikandan Beulaja, Marimuthu Prabhu Narayanan, Raman Thiagarajan, Mahalingam Anjugam, Subramanian Palanisamy, Karthick Saravanan, Munusamy Arumugam
Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
Department of Biochemistry, Annai Veilankanni's College for Women, Chennai 600 015, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5;140:223-8. doi: 10.1016/j.saa.2014.12.060. Epub 2014 Dec 25.
In the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of Solanum trilobatum. The aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. The bio-reduced silver nanoparticles were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX) and X-ray diffraction (XRD). These biologically synthesized silver nanoparticles were tested for its antibacterial activity against few human pathogenic bacteria including Gram-positive (Streptococcus mutans, Enterococcus faecalis) and Gram-negative (Escherichia coli, Klebsiella pneumoniae) bacteria. In addition, we also demonstrated anticancer activity of these nanoparticles in vitro against human breast cancer cell line (MCF 7) using MTT, nuclear morphology assay, Western blot and RT-PCR expression. These results taken together show the potential applications of biosynthesized silver nanoparticles using S. trilobatum fruits.
在本研究中，我们采用一种简单且环保的方法，利用刺天茄未成熟果实合成了银纳米颗粒。当暴露于未成熟果实提取物时，水相银离子被还原并长时间稳定，从而生物合成了表面功能化的银纳米颗粒。通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散光谱（EDX）和X射线衍射（XRD）对生物还原的银纳米颗粒进行了表征。测试了这些生物合成的银纳米颗粒对几种人类病原菌的抗菌活性，包括革兰氏阳性菌（变形链球菌、粪肠球菌）和革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌）。此外，我们还利用MTT、核形态分析、蛋白质免疫印迹和逆转录聚合酶链反应（RT-PCR）表达，在体外证明了这些纳米颗粒对人乳腺癌细胞系（MCF 7）的抗癌活性。综合这些结果表明，利用刺天茄果实生物合成的银纳米颗粒具有潜在的应用价值。
