使用甲醇叶提取物生物合成的蒲公英基纳米颗粒的生物医学潜力。
Biomedical Potentialities of Taraxacum officinale-based Nanoparticles Biosynthesized Using Methanolic Leaf Extract.
作者信息
Rasheed Tahir, Bilal Muhammad, Li Chuanlong, Iqbal Hafiz M N
机构信息
The School of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
出版信息
Curr Pharm Biotechnol. 2017;18(14):1116-1123. doi: 10.2174/1389201019666180214145421.
BACKGROUND
In the present study, the potential of methanolic leaf extract of Taraxacum officinale plant as a function of bio-inspired green synthesis for the fabrication of silver nanoparticles (AgNPs) has been explored.
METHODS
The bio-reduction of aqueous silver nitrate (AgNO3) solution was confirmed by visually detecting the color change from pale yellow to blackish-brown. Maximum absorbance was observed at 420 nm due to the presence of characteristic surface Plasmon resonance of nano silver by UV-visible spectroscopy. The role of various functional groups in the bio-reduction of silver and chemical transformation was verified by Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) predict the shape (rocky, flack type, ellipsoidal, etc.), size (68 nm) and elemental composition (Ag as a major constituent) of the biosynthesized AgNPs, respectively.
RESULTS
Transmission electron microscopy (TEM) analysis further corroborated the morphology of the AgNPs. Color mapping and atomic force microscopy (AFM) confirmed the nano-sized topography. The dynamic light scattering (DLS) showed the charge, stability, and size of the AgNPs. The generated AgNPs presented potential antibacterial activities against Gram-positive and Gram-negative bacterial strains including Staphylococcus aureus, Escherichia coli, and Haemophilus influenzae. The biosynthesized AgNPs also showed antiproliferative activity against MCF-7 breast cancer cell line in a dosedependent manner.
CONCLUSION
In conclusion, results clearly indicate that biosynthesized AgNPs could be used as effective nano drug for treating infectious diseases caused by multidrug resistant bacterial strains in the near future.
背景
在本研究中，已探索了药用蒲公英植物甲醇叶提取物作为生物启发的绿色合成方法用于制备银纳米颗粒（AgNPs）的潜力。
方法
通过肉眼检测硝酸银（AgNO₃）水溶液从浅黄色变为黑褐色的颜色变化来确认生物还原过程。由于纳米银具有特征性表面等离子体共振，通过紫外可见光谱在420nm处观察到最大吸光度。通过傅里叶变换红外光谱（FTIR）验证了各种官能团在银生物还原和化学转化中的作用。扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）分别预测了生物合成的AgNPs的形状（岩石状、片状、椭圆形等）、尺寸（68nm）和元素组成（以银为主要成分）。
结果
透射电子显微镜（TEM）分析进一步证实了AgNPs的形态。颜色映射和原子力显微镜（AFM）证实了纳米级形貌。动态光散射（DLS）显示了AgNPs的电荷、稳定性和尺寸。所生成的AgNPs对包括金黄色葡萄球菌、大肠杆菌和流感嗜血杆菌在内的革兰氏阳性和革兰氏阴性细菌菌株具有潜在的抗菌活性。生物合成的AgNPs还以剂量依赖性方式对MCF-7乳腺癌细胞系显示出抗增殖活性。
结论
总之，结果清楚地表明，在不久的将来，生物合成的AgNPs可作为有效的纳米药物用于治疗由多重耐药细菌菌株引起的传染病。
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