Tu Shu-Ju, Huang Hong-Wen, Chang Wei-Jeng
Department of Medical Imaging and Radiological Sciences, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan.
National Laboratory Animal Center, National Applied Research Laboratories, Nan-Kang, Taipei 115, Taiwan.
Micron. 2015 Apr;71:14-21. doi: 10.1016/j.micron.2014.12.007. Epub 2015 Jan 3.
Aging mice with a rare osteopetrotic disorder in which the entire space of femoral bones are filled with trabecular bones are used as our research platform. A complete study is conducted with a micro computed tomography (CT) system to characterize the bone abnormality. Technical assessment of femoral bones includes geometric structure, biomechanical strength, bone mineral density (BMD), and bone mineral content (BMC). Normal aging mice of similar ages are included for comparisons. In our imaging work, we model the trabecular bone as a cylindrical rod and new quantitative which are not previously discussed are developed for advanced analysis, including trabecular segment length, trabecular segment radius, connecting node number, and distribution of trabecular segment radius. We then identified a geometric characteristic in which there are local maximums (0.0049, 0.0119, and 0.0147 mm) in the structure of trabecular segment radius. Our calculations show 343% higher in percent trabecular bone volume at distal-metaphysis; 38% higher in cortical thickness at mid-diaphysis; 11% higher in cortical cross-sectional moment of inertia at mid-diaphysis; 42% higher in cortical thickness at femur neck; 26% higher in cortical cross-sectional moment of inertia at femur neck; 31% and 395% higher in trabecular BMD and BMC at distal-metaphysis; 17% and 27% higher in cortical BMD and BMC at distal-metaphysis; 9% and 53% higher in cortical BMD and BMC at mid-diaphysis; 25% and 64% higher in cortical BMD and BMC at femur neck. Our new quantitative parameters and findings may be extended to evaluate the treatment response for other similar bone disorders.
患有罕见骨石化症的衰老小鼠被用作我们的研究平台，在这种病症中，股骨的整个空间都充满了小梁骨。使用微型计算机断层扫描（CT）系统进行了一项完整的研究，以表征骨骼异常情况。对股骨的技术评估包括几何结构、生物力学强度、骨矿物质密度（BMD）和骨矿物质含量（BMC）。纳入了年龄相似的正常衰老小鼠进行比较。在我们的成像工作中，我们将小梁骨建模为圆柱形杆，并开发了以前未讨论过的新定量指标用于高级分析，包括小梁段长度、小梁段半径、连接节点数和小梁段半径分布。然后，我们确定了一个几何特征，即在小梁段半径结构中存在局部最大值（0.0049、0.0119和0.0147毫米）。我们的计算表明，远端干骺端的小梁骨体积百分比高343%；骨干中部的皮质厚度高38%；骨干中部的皮质横截面惯性矩高11%；股骨颈的皮质厚度高42%；股骨颈的皮质横截面惯性矩高26%；远端干骺端的小梁BMD和BMC高31%和395%；远端干骺端的皮质BMD和BMC高17%和27%；骨干中部的皮质BMD和BMC高9%和53%；股骨颈的皮质BMD和BMC高25%和64%。我们的新定量参数和发现可能会扩展到评估其他类似骨骼疾病的治疗反应。
