Asgher Muhammad, Yaqoob Mohammad, Nabi Abdul, Murtaza Ghulam, Siddiqi Abdul Rauf, Waseem Amir
Department of Chemistry, University of Balochistan, Sariab Road, Quetta 87300, Pakistan.
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.
Int J Anal Chem. 2014;2014:109592. doi: 10.1155/2014/109592. Epub 2014 Dec 28.
Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB (•-)) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2 (•-)) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals ( (•) OH) were produced via dismutation of H2O2 by catalyst (Cu(2+)). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n = 6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL(-1), respectively. The sample throughput of 70 h(-1) for vitamins A and C and 30 h(-1) for RB was found. Calibration curve was linear in the range of 0.05-15, 0.01-20, and 0.1-50 μg mL(-1) for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n = 3) in the range 1.6-3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.
报道了一种玫瑰红敏化流动注射化学发光法，该方法采用鲁米诺 - 铜（II）测定药物制剂中的维生素A和维生素C。该反应基于分析物对玫瑰红（RB（•-））阴离子自由基产生的增强作用，玫瑰红阴离子自由基与溶解氧迅速相互作用，生成超氧阴离子自由基（O2（•-））和过氧化氢（H2O2）。高活性的羟基自由基（（•）OH）通过催化剂（Cu（2+））使H2O2歧化产生。生成的超氧阴离子自由基和羟基自由基在碱性介质中氧化鲁米诺，产生强烈的化学发光。维生素A、维生素C和玫瑰红的检测限（空白值的3倍标准偏差，n = 6）分别为0.008、0.005和0.05 μg mL-1。发现维生素A和维生素C的样品通量为70 h-1，玫瑰红的样品通量为30 h-1。维生素A、维生素C和玫瑰红的校准曲线分别在0.05 - 15、0.01 - 20和0.1 - 50 μg mL-1范围内呈线性，相对标准偏差（RSDs；n = 3）在1.6 - 3.6%范围内。该方法成功应用于药物制剂，所获结果与标签值吻合良好。
