Department of Chemistry, University of Balochistan, Sariab Road, Quetta, Pakistan.
Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan.
Luminescence. 2023 Jun;38(6):680-691. doi: 10.1002/bio.4495. Epub 2023 Apr 13.
The online photoreaction of the rose bengal photosensitized luminol-copper (II) chemiluminescence (CL) system was used for the determination of β-nicotinamide adenine dinucleotide (NADH) and ethanol (EtOH) in pharmaceutical formulations combined with a flow injection technique. NADH can significantly enhance the CL emission of the reaction. For EtOH, alcohol dehydrogenase in soluble form was utilized in the presence of nicotinamide adenine dinucleotide resulting in NADH production. The limit of detection (3σ blank, 𝑛 = 3) of 4.0 × 10 and 2.17 × 10 M, and linear range 1.3 × 10 to 2.5 × 10 M (R = 0.9998, n = 6) and 0.11-2.17 × 10 M (R = 0.9996, n = 6) were obtained for NADH and EtOH respectively. The injection rate was 100 h with a relative standard deviation (n = 3) of 1.5-4.8% in the range studied for both analytes. The procedure was satisfactorily applied to pharmaceutical formulations with recoveries in the range 91.6 ± 3.0% to 110 ± 2.0% for NADH and 88 ± 3.0% to 95.4 ± 4.0% for EtOH. The results obtained were very consistent and did not differ considerably from the reported approaches at a 95% confidence limit. The possible mechanism of the CL reaction is also explained briefly.
采用在线光化学反应，以玫瑰红 Bengal 敏化鲁米诺-铜（II）化学发光（CL）体系，结合流动注射技术，用于测定药物制剂中的β-烟酰胺腺嘌呤二核苷酸（NADH）和乙醇（EtOH）。NADH 能显著增强反应的 CL 发射。对于 EtOH，在存在烟酰胺腺嘌呤二核苷酸的情况下，利用可溶性形式的醇脱氢酶导致 NADH 的产生。NADH 的检出限（3σ空白，n=3）为 4.0×10 和 2.17×10 ，线性范围为 1.3×10 至 2.5×10 （R = 0.9998，n=6）和 0.11-2.17×10 （R = 0.9996，n=6）。EtOH 的检出限为 1.3×10 至 2.5×10 （R = 0.9998，n=6）和 0.11-2.17×10 （R = 0.9996，n=6）。NADH 和 EtOH 的线性范围分别为 0.11-2.17×10 （R = 0.9996，n=6）。对于两种分析物，在研究范围内，进样速率为 100 h，相对标准偏差（n=3）为 1.5-4.8%。该方法用于药物制剂的测定，回收率在 91.6±3.0%至 110±2.0%范围内，NADH 回收率在 88±3.0%至 95.4±4.0%范围内，EtOH 回收率在 88±3.0%至 95.4±4.0%范围内。结果非常一致，在 95%置信限内与报道的方法没有显著差异。简要解释了 CL 反应的可能机制。
