West Caroline E, Rhodes Blaine N
The Washington State Department of Health, Public Health Laboratories, 1610 NE 150th Street, Shoreline, WA 98155, USA.
Int J Anal Chem. 2014;2014:247316. doi: 10.1155/2014/247316. Epub 2014 Dec 31.
A viable, quick, and reliable method for determining urinary creatinine by liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and used to evaluate spot urine samples collected for the Washington Environmental Biomonitoring Survey (WEBS): part of the Washington State Department of Health, Public Health Laboratories (PHL). 50 µL of urine was mixed with a 1 : 1 acetonitrile/water solution containing deuterated creatinine as the internal standard and then analyzed by LC/MS/MS. Utilizing electrospray ionization (ESI) in positive mode, the transition ions for creatinine and creatinine-d3 were determined to be 114.0 to 44.1 (quantifier), 114.0 to 86.1 (qualifier), and 117.0 to 47.1 (creatinine-d3). The retention time for creatinine was 0.85 minutes. The linear calibration range was 20-4000 mg/L, with a limit of detection at 1.77 mg/L and a limit of quantitation at 5.91 mg/L. LC/MS/MS and the colorimetric Jaffé reaction were associated significantly (Pearson r = 0.9898 and R (2) = 0.9797, ρ ≤ 0.0001). The LC/MS/MS method developed at the PHL to determine creatinine in the spot urine samples had shorter retention times, and was more sensitive, reliable, reproducible, and safer than other LC/MS/MS or commercial methods such as the Jaffé reaction or modified versions thereof.
开发了一种通过液相色谱/串联质谱法（LC/MS/MS）测定尿肌酐的可行、快速且可靠的方法，并用于评估为华盛顿环境生物监测调查（WEBS）采集的随机尿样：该调查是华盛顿州卫生部公共卫生实验室（PHL）的一部分。将50 μL尿液与含有氘代肌酐作为内标的乙腈/水溶液（1∶1）混合，然后通过LC/MS/MS进行分析。在正模式下利用电喷雾电离（ESI），确定肌酐和肌酐-d3的跃迁离子分别为114.0至44.1（定量离子）、114.0至86.1（定性离子）和117.0至47.1（肌酐-d3）。肌酐的保留时间为0.85分钟。线性校准范围为20 - 4000 mg/L，检测限为1.77 mg/L，定量限为5.91 mg/L。LC/MS/MS法与比色法Jaffé反应显著相关（Pearson r = 0.9898，R(2)=0.9797，ρ≤0.0001）。PHL开发的用于测定随机尿样中肌酐的LC/MS/MS方法具有更短的保留时间，并且比其他LC/MS/MS法或商业方法（如Jaffé反应或其改良版本）更灵敏、可靠、可重复且更安全。
