Miralles V J, González-Bosch C, Marcote M J, Hernández-Yago J
Instituto de Investigaciones Citológicas, Centro Asociado del C.S.I.C., Valencia, Spain.
Revis Biol Celular. 1989;21:461-73.
To establish the interrelationship between protein synthesis and degradation, we have developed a simple procedure. We have chosen the mitochondrial proteins, because most of them are synthesized outside mitochondria and are then imported into the organelle. This fact allows to separate easily the synthesis steps from the general turnover. By using this system we have some evidences suggesting that the in vitro protein concentration in mitochondria may be regulated by the entry of mitochondrial protein precursors. Also, we are studying the factors that regulate the import of mitochondrial protein precursors into the organelle, because this step may be essential in the regulation of the turnover of mitochondrial proteins.
为了建立蛋白质合成与降解之间的相互关系，我们开发了一种简单的方法。我们选择了线粒体蛋白，因为它们大多数是在线粒体之外合成的，然后被导入到细胞器中。这一事实使得我们能够轻松地将合成步骤与一般的周转区分开来。通过使用这个系统，我们有一些证据表明线粒体中的体外蛋白质浓度可能受到线粒体蛋白质前体进入的调节。此外，我们正在研究调节线粒体蛋白质前体导入细胞器的因素，因为这一步骤可能在线粒体蛋白质周转的调节中至关重要。
