比索洛尔和氢氯噻嗪二元混合物分光光度法分辨率的比率光谱不同信号处理技术；一项比较研究。
Different signal processing techniques of ratio spectra for spectrophotometric resolution of binary mixture of bisoprolol and hydrochlorothiazide; a comparative study.
作者信息
Elzanfaly Eman S, Hassan Said A, Salem Maissa Y, El-Zeany Badr A
机构信息
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5;140:334-43. doi: 10.1016/j.saa.2014.12.107. Epub 2015 Jan 3.
Five signal processing techniques were applied to ratio spectra for quantitative determination of bisoprolol (BIS) and hydrochlorothiazide (HCT) in their binary mixture. The proposed techniques are Numerical Differentiation of Ratio Spectra (ND-RS), Savitsky-Golay of Ratio Spectra (SG-RS), Continuous Wavelet Transform of Ratio Spectra (CWT-RS), Mean Centering of Ratio Spectra (MC-RS) and Discrete Fourier Transform of Ratio Spectra (DFT-RS). The linearity of the proposed methods was investigated in the range of 2-40 and 1-22 μg/mL for BIS and HCT, respectively. The proposed methods were applied successfully for the determination of the drugs in laboratory prepared mixtures and in commercial pharmaceutical preparations and standard deviation was less than 1.5. The five signal processing techniques were compared to each other and validated according to the ICH guidelines and accuracy, precision, repeatability and robustness were found to be within the acceptable limit.
采用五种信号处理技术对比光谱进行处理，用于定量测定比索洛尔（BIS）和氢氯噻嗪（HCT）二元混合物中的这两种成分。所提出的技术包括比光谱数值微分（ND-RS）、比光谱Savitsky-Golay变换（SG-RS）、比光谱连续小波变换（CWT-RS）、比光谱均值中心化（MC-RS）和比光谱离散傅里叶变换（DFT-RS）。分别考察了所提出方法在2 - 40 μg/mL（针对BIS）和1 - 22 μg/mL（针对HCT）范围内的线性。所提出的方法成功应用于实验室配制混合物和市售药物制剂中药物的测定，标准偏差小于1.5。对这五种信号处理技术进行了相互比较，并根据ICH指南进行了验证，发现准确度、精密度、重复性和稳健性均在可接受范围内。
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