Attia Khalid A M, Nassar Mohammed W I, El-Zeiny Mohamed B, Serag Ahmed
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582 Al Hadaba Al Wosta, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Feb 5;154:232-236. doi: 10.1016/j.saa.2015.10.040. Epub 2015 Oct 26.
Four rapid, simple, accurate and precise spectrophotometric methods were used for the determination of ciprofloxacin in the presence of metronidazole as interference. The methods under study are area under the curve, simultaneous equation in addition to smart signal processing techniques of manipulating ratio spectra namely Savitsky-Golay filters and continuous wavelet transform. All the methods were validated according to the ICH guidelines where accuracy, precision and repeatability were found to be within the acceptable limits. The selectivity of the proposed methods was tested using laboratory prepared mixtures and assessed by applying the standard addition technique. So, they can therefore be used for the routine analysis of ciprofloxacin in quality-control laboratories.
采用四种快速、简单、准确且精密的分光光度法，在甲硝唑存在干扰的情况下测定环丙沙星。所研究的方法有曲线下面积法、联立方程法，以及用于处理比率光谱的智能信号处理技术，即Savitsky-Golay滤波器和连续小波变换。所有方法均按照国际人用药品注册技术协调会（ICH）指南进行验证，结果发现准确度、精密度和重复性均在可接受范围内。使用实验室配制的混合物对所提出方法的选择性进行了测试，并通过标准加入法进行评估。因此，它们可用于质量控制实验室中环丙沙星的常规分析。
