a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
Artif Cells Nanomed Biotechnol. 2016 May;44(3):943-9. doi: 10.3109/21691401.2014.1001493. Epub 2015 Jan 23.
Magnetic iron oxide nanoparticles (IONPs) were coated with gelatin type B by means of the two-step desolvation method. Drug loading by adsorption was studied under various conditions such as different temperature, contact time, pH, and initial gemcitabine concentration. Further, Langmuir isotherm curves were constracted and constants were calculated. According to the Langmuir isotherm, the Gibbs free energy of the adsorption process at 25°C was - 4.74 kJ/mol. On the other hand, this value at 37°C was - 7.86 kJ/mol. In vitro drug release was performed at pH levels of 5 and 7.4, with gemcitabine-loaded magnetic gelatin nanoparticles and free gemcitabine, and both the results were subsequently compared.
磁性氧化铁纳米粒子（IONPs）通过两步去溶剂化法用明胶 B 型进行了包覆。通过吸附作用在不同条件下（如不同的温度、接触时间、pH 值和初始吉西他滨浓度）研究了载药情况。进一步构建了 Langmuir 等温线并计算了常数。根据 Langmuir 等温线，在 25°C 下吸附过程的吉布斯自由能为-4.74 kJ/mol。另一方面，在 37°C 下该值为-7.86 kJ/mol。在 pH 值为 5 和 7.4 下进行了载吉西他滨磁性明胶纳米粒子和游离吉西他滨的体外药物释放，并对这两种结果进行了比较。
