Lin Li-Li, Wang Dong, Wang Wei, Cheng Yan-Qiong, Su Ding-Feng, Liu Ai-Jun
Department of Pharmacology, Wuxi Higher Health Vocational Technology School, Wuxi, Jiangsu, China; Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China; Department of Pharmacy Administration, Chinese PLA General Hospital, Beijing, China.
PLoS One. 2015 Jan 23;10(1):e0116155. doi: 10.1371/journal.pone.0116155. eCollection 2015.
This study was designed to investigate the impact of representative antihypertensive drugs of 5 classes on the sexual function in male spontaneously hypertensive rats (SHR) at doses that achieved similar blood pressure (BP) reduction. The experiment was performed in 6 groups of male SHR. The dose are 20 μg/kg/day for clonidine, 3 mg/kg/day for enalapril, 20 mg/kg/day for atenolol, 2 mg/kg/day for amlodipine, and 10 mg/kg/day for dihydrochlorothiazide. SHR were treated for 3 months, and then the penile erection and sexual behavior were detected. After BP recording, SHR were killed to evaluate the organ-damage, weight of accessory sex organs and levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone in serum. Five drugs had the similar efficacy on BP reduction. All drugs except of enalapril, significantly prolonged the mount latency, and decreased the mount frequency (P<0.05). Clonidine also reduced the conception rate (45% vs. 80% in control group, P<0.05). Amlodipine and dihydrochlorothiazide significantly increased the testosterone level (0.79±0.30, 0.80±0.34 vs. 0.49±0.20 in control group, unit: ng/dl, P<0.05). Enalapril, atenolol and amlodipine also significantly decreased the BP variability (systolic, 8.2±2.5, 7.6±1.8, 8.9±2.0 vs. 12.2±3.8 in control group, unit: mm Hg). All these drugs significantly decreased the organ-damage (P<0.05). In conclusion, long-term treatment with 5 common antihypertensive drugs possessed obvious organ protection in SHR. Clonidine, atenolol, amlodipine and dihydrochlorothiazide, but not enalapril, impair sexual function.
本研究旨在探讨5类代表性抗高血压药物在实现相似血压降低剂量时对雄性自发性高血压大鼠（SHR）性功能的影响。实验在6组雄性SHR中进行。剂量分别为可乐定20μg/kg/天、依那普利3mg/kg/天、阿替洛尔20mg/kg/天、氨氯地平2mg/kg/天和氢氯噻嗪10mg/kg/天。对SHR进行3个月治疗，然后检测阴茎勃起和性行为。记录血压后，处死SHR以评估器官损伤、附属生殖器官重量以及血清中促卵泡激素（FSH）、黄体生成素（LH）和睾酮水平。5种药物在降低血压方面具有相似疗效。除依那普利外，所有药物均显著延长了骑跨潜伏期，并降低了骑跨频率（P<0.05）。可乐定还降低了受孕率（对照组为80%，可乐定组为45%，P<0.05）。氨氯地平和氢氯噻嗪显著提高了睾酮水平（对照组为0.49±0.20，氨氯地平组为0.79±0.30，氢氯噻嗪组为0.80±0.34，单位：ng/dl，P<0.05）。依那普利、阿替洛尔和氨氯地平也显著降低了血压变异性（收缩压，对照组为12.2±3.8，依那普利组为8.2±2.5，阿替洛尔组为7.6±1.8，氨氯地平组为8.9±2.0，单位：mmHg）。所有这些药物均显著降低了器官损伤（P<0.05）。总之，5种常用抗高血压药物长期治疗对SHR具有明显的器官保护作用。可乐定、阿替洛尔、氨氯地平和氢氯噻嗪会损害性功能，而依那普利不会。
