Erol Öztürk Yeter, Yeter Oya, Alpertunga Buket
Council of Forensic Medicine, Bursa Chemistry Department, Toxicology Division, 16140 Ataevler, Bursa, Turkey.
Council of Forensic Medicine, Istanbul Chemistry Department, Toxicology Division, 34196 Bahcelievler, Istanbul, Turkey.
Forensic Sci Int. 2015 Mar;248:88-93. doi: 10.1016/j.forsciint.2014.12.029. Epub 2015 Jan 9.
The herbal products referred to as 'Spice' have been used as 'legal alternatives' to cannabis worldwide since 2004. The first synthetic cannabinoid JWH-018 was detected in 'Spice' products in 2008, and has been banned by many legal authorities since the beginning of 2009. In order to prove use of JWH cannabinoids (JWHs), specific and robust methods were needed. We have developed a specific and reliable method for the detection and quantification of JWH-018, JWH-018 N-pentanoic acid, and JWH-018 N-(5-hydroxypentyl) in blood and urine using solid-phase extraction followed by UPLC-MS/MS analysis. The method has been validated in terms of linearity (0.1-50ng/mL), selectivity, intra-assay and inter-assay accuracy and precision (CV<15%), recovery (85-98%), limits of detection (LOD) (0.08-0.14ng/mL), and quantification (LOQ) (0.10-0.21ng/mL). Matrix effects, stability, and process efficiency were also assessed. The method has been applied to 868 authentic samples received by the Department of Chemistry (Istanbul) in the Council of Forensic Medicine of the Ministry of Justice.
自2004年以来，被称为“香料”的草药产品在全球范围内被用作大麻的“合法替代品”。2008年在“香料”产品中检测到第一种合成大麻素JWH-018，自2009年初以来已被许多法律当局禁止。为了证明JWH大麻素（JWHs）的使用情况，需要特定且可靠的方法。我们开发了一种特定且可靠的方法，用于通过固相萃取然后进行超高效液相色谱-串联质谱（UPLC-MS/MS）分析，检测和定量血液及尿液中的JWH-018、JWH-018 N-戊酸和JWH-018 N-(5-羟基戊基)。该方法已在以下方面得到验证：线性（0.1-50ng/mL）、选择性、批内和批间准确度及精密度（CV<15%）、回收率（85-98%）、检测限（LOD）（0.08-0.14ng/mL）和定量限（LOQ）（0.10-0.21ng/mL）。还评估了基质效应、稳定性和处理效率。该方法已应用于司法部法医委员会化学部（伊斯坦布尔）收到的868份真实样本。
