Chandran Manju, Chu Ye, Maleki Soheila J, Ozias-Akins Peggy
Department of Horticulture, University of Georgia , Tifton, Georgia 31793-5766, United States.
J Agric Food Chem. 2015 Feb 18;63(6):1788-97. doi: 10.1021/jf504892f. Epub 2015 Feb 9.
Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T3, T4, and T5) under field conditions. Different soil sulfur levels (0.012, 0.3, and 3.0 mM) differentially impacted sulfur-rich (Ara h 2, Ara h 3, and Ara h 6) versus sulfur-poor (Ara h 1) proteins in non-transgenic versus transgenic peanut. The sulfur level had no effect on Ara h 1, whereas low sulfur led to a significant reduction of Ara h 3 in transgenic and non-transgenic seeds and Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanuts because these proteins already were reduced by gene silencing. These results demonstrate stability of transgene expression and the potential utility of RNAi in allergen manipulation.
含有用于RNA干扰（RNAi）设计基因的转基因花生（Arachis hypogaea L.）在田间条件下的三代（T3、T4和T5）中，显示出Ara h 2的稳定完全沉默以及Ara h 6的部分沉默，Ara h 2和Ara h 6是两种强效花生过敏原/蛋白质，同时对其他过敏原Ara h 1和Ara h 3的附带变化最小。不同土壤硫水平（0.012、0.3和3.0 mM）对非转基因和转基因花生中富含硫的（Ara h 2、Ara h 3和Ara h 6）与贫硫的（Ara h 1）蛋白质有不同影响。硫水平对Ara h 1没有影响，而低硫导致转基因和非转基因种子中Ara h 3显著减少，非转基因花生中Ara h 2和Ara h 6显著减少，但转基因花生中未减少，因为这些蛋白质已经通过基因沉默而减少。这些结果证明了转基因表达的稳定性以及RNAi在过敏原操纵中的潜在用途。
