Chu Ye, Faustinelli Paola, Ramos Maria Laura, Hajduch Martin, Stevenson Severin, Thelen Jay J, Maleki Soheila J, Cheng Hsiaopo, Ozias-Akins Peggy
Department of Horticulture, University of Georgia, 31793, USA.
J Agric Food Chem. 2008 Dec 10;56(23):11225-33. doi: 10.1021/jf802600r.
The most potent peanut allergens, Ara h 2 and Ara h 6, were silenced in transgenic plants by RNA interference. Three independent transgenic lines were recovered after microprojectile bombardment, of which two contained single, integrated copies of the transgene. The third line contained multiple copies of the transgene. Ara h 2 expression was significantly suppressed in all three lines, whereas Ara h 6 was reduced in two lines. Expression of peanut allergens Ara h 1 and Ara h 3 was not noticeably affected. Significant reduction of human IgE binding to Ara h 2 and Ara h 6 also was observed. Seed weight and germination data from transgenic and nontransgenic segregants showed no significant differences. Data collected from in vitro Aspergillus flavus infection indicate no significant difference in fungal growth between the transgenic lines and the nontransgenic controls. These data suggest that silencing Ara h 2 and Ara h 6 is a feasible approach to produce hypoallergenic peanut.
通过RNA干扰使转基因植物中最具活性的花生过敏原Ara h 2和Ara h 6沉默。经微粒轰击后获得了三个独立的转基因株系，其中两个含有单个整合的转基因拷贝。第三个株系含有多个转基因拷贝。在所有三个株系中Ara h 2的表达均被显著抑制，而在两个株系中Ara h 6有所减少。花生过敏原Ara h 1和Ara h 3的表达未受到明显影响。还观察到人类IgE与Ara h 2和Ara h 6的结合显著减少。转基因和非转基因分离株的种子重量和发芽数据没有显著差异。从体外黄曲霉感染收集的数据表明，转基因株系与非转基因对照之间的真菌生长没有显著差异。这些数据表明，使Ara h 2和Ara h 6沉默是生产低过敏性花生的一种可行方法。
