Omoto E, Tavassoli M
Veterans Administration Medical Center, Jackson, MS.
Trans Assoc Am Physicians. 1989;102:170-5.
Membrane receptors for ceruloplasmin were isolated by affinity chromatography using CP bound to CNBr-activated Sepharose 4B. The receptor was obtained in the form of a single sharp peak which when collected, concentrated and run on SDS-PAGE provided a single band with a molecular weight of 35,000. Treatment with endoglycosidase F reduced this molecular weight by 3%. It is concluded that ceruloplasmin receptors in this cell system is a membrane protein containing 3% carbohydrate with a total molecular weight of 35,000. It is possible that this is a monomer of a higher molecular weight protein.
使用结合到溴化氰活化的琼脂糖凝胶4B上的铜蓝蛋白，通过亲和色谱法分离铜蓝蛋白的膜受体。以单个尖锐峰的形式获得该受体，收集、浓缩并在SDS-PAGE上运行时，得到一条分子量为35,000的单带。用内切糖苷酶F处理使该分子量降低了3%。得出结论，该细胞系统中的铜蓝蛋白受体是一种膜蛋白，含有3%的碳水化合物，总分子量为35,000。这有可能是一种高分子量蛋白质的单体。
