Chen Jiaxiang, Zhou Hao, Wang Jinlei, Zhang Baoping, Liu Fen, Huang Jian, Li Jia, Lin Jiari, Bai Jiali, Liu Renping
Medical Experiment Education Department, Medical College of Nanchang University, Nanchang 330031, China.
Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
Int Immunopharmacol. 2015 Mar;25(1):43-8. doi: 10.1016/j.intimp.2015.01.013. Epub 2015 Jan 20.
Asthma is an inflammatory disease of the lungs characterized by airway remolding. In this study, we examined whether resveratrol exerts protective effects on allergic asthma in a murine model. To investigate the effects of resveratrol on allergic airway inflammation in house dust mite (HDM)-induced mouse asthma and explore its mechanism, a chronic asthma mouse model was established by intranasally administering extracts of HDM (25μg of protein in 10μl of saline) for 5days/week for up to 7 consecutive weeks. Resveratrol (50mg/kg body weight), dexamethasone (1mg/kg body weight) or a vehicle was administered orally 1h before antigen challenges for up to 2weeks. Compared with the HDM-induced mice, the level of TNF-α of the BALF in the resveratrol+HDM-treated mice had obviously decreased. Histological examination of the lung tissue revealed that the resveratrol treatments attenuated the fibrotic response and airway inflammation. In addition, resveratrol inhibited the expression of the Syk protein and degranulation in mast cells. The presented findings collectively suggest that resveratrol has a therapeutic effect on mouse allergic asthma, and its mechanism of action might be related to reducing the production of the Syk protein.
哮喘是一种以气道重塑为特征的肺部炎症性疾病。在本研究中，我们检测了白藜芦醇在小鼠模型中对过敏性哮喘是否具有保护作用。为了研究白藜芦醇对屋尘螨（HDM）诱导的小鼠哮喘中过敏性气道炎症的影响并探究其机制，通过每周5天、连续7周经鼻给予HDM提取物（10μl盐水中含25μg蛋白质）建立慢性哮喘小鼠模型。在抗原激发前1小时口服给予白藜芦醇（50mg/kg体重）、地塞米松（1mg/kg体重）或赋形剂，持续2周。与HDM诱导的小鼠相比，白藜芦醇+HDM处理组小鼠支气管肺泡灌洗液（BALF）中TNF-α水平明显降低。肺组织的组织学检查显示，白藜芦醇处理减轻了纤维化反应和气道炎症。此外，白藜芦醇抑制了脾酪氨酸激酶（Syk）蛋白的表达以及肥大细胞的脱颗粒。这些研究结果共同表明，白藜芦醇对小鼠过敏性哮喘具有治疗作用，其作用机制可能与减少Syk蛋白的产生有关。
