Pröls Felicitas, Wiegreffe Christoph, Scaal Martin
Institute of Anatomy II, Department of Vertebrate Embryology, University of Cologne, Joseph-Stelzmann-Straße 9, Cologne 50931, Germany Institute of Anatomy and Cell Biology, Department of Molecular Embryology, University of Freiburg, Albertstraße 17, Freiburg 79104, Germany Faculty of Biology, University of Freiburg, Schaenzlestraße 1, Freiburg 79104, Germany.
Institute of Anatomy II, Department of Vertebrate Embryology, University of Cologne, Joseph-Stelzmann-Straße 9, Cologne 50931, Germany.
Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. Epub 2015 Jan 23.
Long-range intercellular communication is essential for the regulation of embryonic development. Apart from simple diffusion, various modes of signal transfer have been described in the literature. Here, we describe a novel type of cellular extensions found in epithelial cells of the somites in chicken embryos. These filopodia-like protrusions span the subectodermal space overlying the dorsal surface of the somites and contact the ectoderm. We show that these protrusions are actin- and tubulin-positive and require Rac1 for their formation. The presence of glycophosphatidylinositol-anchored proteins and net retrograde trafficking of the transmembrane Wnt-receptor Frizzled-7 along the protrusions indicate their role in signal transport and distribution. Taken together, our data suggest a role of filopodia-like protrusions in mediating signaling events between distant epithelial cells during embryonic development.
长距离细胞间通讯对于胚胎发育的调控至关重要。除了简单扩散外，文献中还描述了多种信号传递模式。在此，我们描述了在鸡胚体节上皮细胞中发现的一种新型细胞延伸结构。这些丝状伪足样突起跨越覆盖体节背表面的皮下空间并与外胚层接触。我们发现这些突起对肌动蛋白和微管蛋白呈阳性反应，且其形成需要Rac1。糖基磷脂酰肌醇锚定蛋白的存在以及跨膜Wnt受体卷曲蛋白7沿突起的净逆行运输表明它们在信号转运和分布中的作用。综上所述，我们的数据表明丝状伪足样突起在胚胎发育过程中介导远距离上皮细胞之间的信号传导事件中发挥作用。
