使用在固体培养基上生长的拟南芥幼苗进行化学遗传学筛选。

Chemical genetic screens using Arabidopsis thaliana seedlings grown on solid medium.

作者信息

Dinh Thanh Theresa, Chen Xuemei

机构信息

Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.

出版信息

Methods Mol Biol. 2015;1263:111-25. doi: 10.1007/978-1-4939-2269-7_9.

DOI:10.1007/978-1-4939-2269-7_9

PMID:25618340

Abstract

Genetic screening has been a powerful tool in identifying new genes in a pathway of interest (forward genetics) or attributing function to a particular gene via mutagenesis (reverse genetics). Small molecule-based chemical genetics is increasingly adapted in Arabidopsis research as a tool for similar purposes, i.e., to identify genes involved in certain biological processes and to dissect the biological roles of a gene. Chemical genetic screens have been successful in circumventing genetic redundancy to assign biological roles to a gene family as well as novel functions for well-known genes. Here, we describe how to screen Arabidopsis seedlings grown on solid medium with chemical compounds.

摘要

遗传筛选一直是一种强大的工具，可用于在感兴趣的途径中鉴定新基因（正向遗传学），或通过诱变将功能归因于特定基因（反向遗传学）。基于小分子的化学遗传学在拟南芥研究中越来越多地被用作类似目的的工具，即鉴定参与某些生物学过程的基因并剖析基因的生物学作用。化学遗传筛选已成功规避遗传冗余，从而为基因家族赋予生物学作用，并为知名基因赋予新功能。在此，我们描述了如何用化合物筛选在固体培养基上生长的拟南芥幼苗。