Department of Microbial Pathogenesis, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT, USA.
Yale Systems Biology Institute, Yale University, West Haven, CT, USA.
Methods Mol Biol. 2021;2213:29-37. doi: 10.1007/978-1-0716-0954-5_3.
Forward genetics has been extremely powerful for dissecting biological pathways in various model organisms. However, it is limited by the fact that redundant gene families and essential genes cannot be readily uncovered through such methods. Chemical genetics, on the other hand, provides a valuable complementary approach to probe biological processes and is suitable for not only genetic model organisms but also genetically less tractable species. We describe here a high-throughput chemical genetic screening method simply based on plant growth and developmental phenotypes in Arabidopsis. It was successfully utilized to study plant immunity and can be easily adapted for dissecting other plant signal transduction pathways.
正向遗传学在解析各种模式生物的生物途径方面非常强大。然而，它受到冗余基因家族和必需基因无法通过这种方法轻易揭示的事实的限制。另一方面，化学遗传学为探究生物过程提供了一种有价值的互补方法，不仅适用于遗传模式生物，也适用于遗传上较难处理的物种。我们在这里描述了一种基于拟南芥生长和发育表型的高通量化学遗传筛选方法。它已成功用于研究植物免疫，并且可以很容易地适应于解析其他植物信号转导途径。
