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长期监测分子标志物可以区分粪便指示细菌来源的不同季节性模式。

Long-term monitoring of molecular markers can distinguish different seasonal patterns of fecal indicating bacteria sources.

机构信息

Department of Civil and Environmental Engineering, University of California Los, Angeles, Los Angeles, CA 90095, USA.

Department of Civil and Environmental Engineering, University of California Los, Angeles, Los Angeles, CA 90095, USA.

出版信息

Water Res. 2015 Mar 15;71:227-43. doi: 10.1016/j.watres.2014.12.037. Epub 2014 Dec 30.

DOI:10.1016/j.watres.2014.12.037
PMID:25618519
Abstract

Elevated levels of fecal indicator bacteria (FIB) have been observed at Topanga Beach, CA, USA. To identify the FIB sources, a microbial source tracking study using a dog-, a gull- and two human-associated molecular markers was conducted at 10 sites over 21 months. Historical data suggest that episodic discharge from the lagoon at the mouth of Topanga Creek is the main source of bacteria to the beach. A decline in creek FIB/markers downstream from upper watershed development and a sharp increase in FIB/markers at the lagoon sites suggest sources are local to the lagoon. At the lagoon and beach, human markers are detected sporadically, dog marker peaks in abundance mid-winter, and gull marker is chronically elevated. Varied seasonal patterns of FIB and source markers were identified showing the importance of applying a suite of markers over long-term spatial and temporal sampling to identify a complex combination of sources of contamination.

摘要

美国加利福尼亚州托潘加海滩的粪便指示菌(FIB)水平升高。为了确定 FIB 的来源,在 21 个月的时间里,在 10 个地点进行了一项使用与狗、海鸥和两个人类相关的分子标记物的微生物来源追踪研究。历史数据表明,托潘加溪河口泻湖的间歇性排放是细菌进入海滩的主要来源。在上游集水区开发后,溪流 FIB/标记物的减少以及泻湖地点 FIB/标记物的急剧增加表明,污染源就在泻湖附近。在泻湖和海滩,人类标记物偶有检出,狗标记物在冬季中期大量出现,海鸥标记物持续升高。确定了 FIB 和来源标记物的不同季节模式,表明应用一套标记物进行长期的空间和时间采样对于识别复杂的污染来源组合非常重要。

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