电化学测定抗癌药物紫杉醇在 ds-DNA 修饰的铅笔状石墨电极上的电化学行为及其作为无标记电化学生物传感器的应用。
Electrochemical determination of the anticancer drug taxol at a ds-DNA modified pencil-graphite electrode and its application as a label-free electrochemical biosensor.
机构信息
Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran; Young Researchers Society, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
出版信息
Talanta. 2015 Mar;134:60-64. doi: 10.1016/j.talanta.2014.10.063. Epub 2014 Nov 10.
In this study a novel biosensor for determination of taxol is described. The interaction of taxol with salmon-sperm double-stranded DNA (ds-DNA) based on the decreasing of the oxidation signals of guanine and adenine bases was studied electrochemically with a pencil-graphite electrode (PGE) using a differential pulse voltammetry (DPV) method. The decreases in the intensity of the guanine and adenine oxidation signals after interaction with taxol were used as indicator signals for the sensitive determination of taxol. DPV exhibits a linear dynamic range of 2.0×10(-7)-1.0×10(-5) M for taxol with a detection limit of 8.0×10(-8) M. Finally, this modified electrode was used for determination of taxol in some real samples.
本研究描述了一种用于测定紫杉醇的新型生物传感器。本文用电化学方法，利用差分脉冲伏安法（DPV），以鲑鱼精双链 DNA（ds-DNA）与紫杉醇相互作用导致鸟嘌呤和腺嘌呤碱基氧化信号减弱为基础，研究了紫杉醇与鲑鱼精 ds-DNA 的相互作用。紫杉醇与 ds-DNA 相互作用后，鸟嘌呤和腺嘌呤氧化信号强度的降低可作为灵敏测定紫杉醇的指示信号。DPV 对紫杉醇的线性动态范围为 2.0×10(-7)-1.0×10(-5) M，检测限为 8.0×10(-8) M。最后，该修饰电极用于测定一些实际样品中的紫杉醇。
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