Wu Grace, Zaman Muhammad H
Department of Biomedical Engineering, Boston University, Boston, MA, 02215.
Department of Biomedical Engineering, Boston University, Boston, MA, 02215.
Talanta. 2015 Mar;134:194-199. doi: 10.1016/j.talanta.2014.10.061. Epub 2014 Nov 7.
Recent advances in electrochemical analysis on filter paper exemplify the versatility of this substrate for high performance testing. Its low-cost, light-weight, and environmentally friendly properties make it particularly attractive for applications in addressing health and environmental safety needs in low-resource settings and developing countries. However, the main drawback to sensitive electrochemical testing is the use of a potentiostat, a bench-top instrument that is extremely expensive, thereby negating the some of the benefits of paper-based devices. Hence there is a need to develop paper-devices for use with handheld, portable device readers that can extract quantitative readouts. In this study, we developed a method to use micro-paper electrochemical devices, or µPEDs, with a glucose meter, which are used for personal monitoring of blood glucose levels. Ethanol was chosen as a model target analyte due to its importance in the global issue of road safety. µPEDs were simple in design and could be tested with a potentiostat. We observed that inclusion of the stabilizer trehalose was critical to preparing µPEDs for later analysis. In addition, an NAD(+)-dependent enzyme was used to impart selectivity to the biosensor, which also represents a class of enzymes with targets relevant to the health and food industry.
滤纸电化学分析的最新进展体现了这种基质在高性能测试方面的多功能性。其低成本、轻质和环保特性使其在满足资源匮乏地区和发展中国家的健康与环境安全需求的应用中特别具有吸引力。然而，灵敏电化学测试的主要缺点是使用恒电位仪，这是一种极为昂贵的台式仪器，从而抵消了纸质设备的一些优势。因此，需要开发与手持式便携式设备读取器配合使用的纸质设备，以便能够提取定量读数。在本研究中，我们开发了一种方法，将微纸电化学装置（µPEDs）与血糖仪一起使用，血糖仪用于个人血糖水平监测。由于乙醇在全球道路安全问题中的重要性，我们选择乙醇作为模型目标分析物。µPEDs设计简单，可用恒电位仪进行测试。我们观察到，加入稳定剂海藻糖对于制备用于后续分析的µPEDs至关重要。此外，使用了一种依赖烟酰胺腺嘌呤二核苷酸（NAD(+)）的酶来赋予生物传感器选择性，这类酶的靶点也与健康和食品行业相关。
