Albrecht R, Verrecchia E, Pfeifer H-R
Institut des Sciences de la Terre, Université de Lausanne, Géopolis, CH-1015 Lausanne, Switzerland; Laboratory of Ecological Systems (ECOS), School of Architecture, Civil and Environmental Engineering (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 2, 1015, Lausanne, Switzerland; Research Unit Community Ecology, WSL Swiss Federal Institute for Forest, Snow and Landscape Research, Site Lausanne, Station 2, 1015, Lausanne, Switzerland.
Institut des Sciences de la Terre, Université de Lausanne, Géopolis, CH-1015 Lausanne, Switzerland.
Talanta. 2015 Mar;134:453-459. doi: 10.1016/j.talanta.2014.11.056. Epub 2014 Dec 3.
Given its high sensitivity and non-destructive nature, fluorescence excitation-emission matrix (EEM) spectroscopy is widely used to differentiate changes and transformations of dissolved or water-extracted organic matter (OM) in natural environments. The same technique applied directly on solid samples (solid-phase fluorescence spectroscopy, SPF-EEM) provides accurate results when used with pharmaceutical products or food samples, but only a few studies have considered natural OM. This study reports on the use of SPF-EEM on solid compost samples and emphasises the way the different maturation phases can be distinguished with fluorophores closely resembling those found in dissolved samples. A very good correlation has been found with data from Rock-Eval pyrolysis, nuclear magnetic resonance ((13)C CPMAS NMR), and humic-fulvic acid ratios determined by conventional NaOH-extraction. SPF-EEM appears as a much simpler method than the conventional ones to detect transformations in natural OM samples with low mineral contents. However, direct application to soil samples requires some additional studies.
鉴于其高灵敏度和非破坏性，荧光激发-发射矩阵（EEM）光谱法被广泛用于区分自然环境中溶解或水提取的有机物质（OM）的变化和转化。当应用于固体样品时（固相荧光光谱法，SPF-EEM），该技术与药品或食品样品一起使用时可提供准确的结果，但只有少数研究考虑了天然有机物质。本研究报告了在固体堆肥样品上使用SPF-EEM的情况，并强调了通过与溶解样品中发现的荧光团非常相似的荧光团来区分不同成熟阶段的方法。已发现与岩石热解评价、核磁共振（（13）C CPMAS NMR）以及通过传统NaOH萃取测定的腐殖酸-富里酸比率的数据具有很好的相关性。与传统方法相比，SPF-EEM似乎是一种更简单的方法，用于检测低矿物质含量的天然有机物质样品中的转化。然而，直接应用于土壤样品还需要一些额外的研究。
