Wang Wandan, Wang Lan, Yang Hongjun, Wang Jianlu, Yin Xiaojie, Xu Haiyu, Cheng Long, Liang Rixin
J Tradit Chin Med. 2014 Dec;34(6):699-709. doi: 10.1016/s0254-6272(15)30085-6.
To investigate the effects of Yindanxinnaotong capsule (YDXNTC) and main components compatibility and ratios on myocardium against ischemia/reperfusion injury and the effect's underlying mechanism.
Myocardial ischemia/reperfusion injury (MIRI) was induced by ischemia for 30 min and reperfusion for 30 min. Electrocardiogram data and coronary flow were recorded, and superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase, creatine kinase-MB, cardiac troponin T and I (cTnT, cTnI) and interleukin-1β, interleukin-8, interleukin-18 (IL-1β, IL-8, IL-18) in myocardium were measured. Hypoxia/reoxygenation and hydrogen peroxide (H2O2) injury were induced by hypoxia for 3 h/reoxygenation for 2 h, and 100 μM H2O2 for 1 h, respectively, in vitro rat myocardial cells (H9c2). Cell viability, SOD, MDA, cTnT and inflammatory factors (IL-1β, IL-8 and IL-18) were determined, and Toll-like receptor 4 (TLR-4) expression was measured by western blotting.
In the isolated heart experiment, elevated heart function, coronary flow and SOD levels, and decreased MDA levels and inflammatory factors were noted in the YDXNTC, main components and main components compatibility groups. Ventricular tachycardia/ventricular fibrillation occurrence decreased in the ginkgo biloba extract (GBE), and GBE and salvia miltiorrhiza ethanol extract compatibility (SM-E, GSEC) groups. Lactic dehydrogenase levels decreased in the YDXNTC and aqueous extract of salvia miltiorrhiza (SM-H) groups. Creatine kinase-MB decreased with GBE, SM-E, SM-H and GSEC treatment, and cTnI and cTnT levels decreased with GSEC. In the in vitro cell study, YDXNTC and main components ratios improved cell viability and SOD levels, and suppressed MDA, cTnT and inflammatory factors. TLR-4 expression was down-regulated.
YDXNTC and main components compatibility showed protective effects on MIRI in this rat model and in vitro study. Regulating the Toll-like receptor signaling pathway may affect the mechanism.
探讨银丹心脑通软胶囊（YDXNTC）及其主要成分的配伍和比例对心肌缺血/再灌注损伤的影响及其作用机制。
采用缺血30分钟再灌注30分钟诱导心肌缺血/再灌注损伤（MIRI）。记录心电图数据和冠脉流量，并测定心肌中超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶、肌酸激酶同工酶MB、心肌肌钙蛋白T和I（cTnT、cTnI）以及白细胞介素-1β、白细胞介素-8、白细胞介素-18（IL-1β、IL-8、IL-18）。分别在体外大鼠心肌细胞（H9c2）中通过缺氧3小时/复氧2小时以及100 μM过氧化氢（H2O2）处理1小时诱导缺氧/复氧和H2O2损伤。测定细胞活力、SOD、MDA、cTnT和炎症因子（IL-1β、IL-8和IL-18），并通过蛋白质印迹法检测Toll样受体4（TLR-4）的表达。
在离体心脏实验中，YDXNTC组、主要成分组和主要成分配伍组的心功能、冠脉流量和SOD水平升高，MDA水平和炎症因子降低。银杏叶提取物（GBE）组以及GBE与丹参乙醇提取物配伍（SM-E、GSEC）组室性心动过速/心室颤动的发生率降低。YDXNTC组和丹参水提取物（SM-H）组的乳酸脱氢酶水平降低。GBE、SM-E、SM-H和GSEC处理后肌酸激酶同工酶MB降低，GSEC处理后cTnI和cTnT水平降低。在体外细胞研究中，YDXNTC及其主要成分比例提高了细胞活力和SOD水平，并抑制了MDA、cTnT和炎症因子。TLR-4表达下调。
在本大鼠模型和体外研究中，YDXNTC及其主要成分配伍对MIRI具有保护作用。调节Toll样受体信号通路可能影响其作用机制。
