Hu Yan-wu, Liu Kai, Yan Meng-tong
Zhongguo Zhong Yao Za Zhi. 2015 Nov;40(21):4234-9.
To study the therapeutic effect and possible mechanism of icariin on myocardial ischemia-reperfusion injury ( MIRI) model in diabetes rats. The model of diabetic rats were induced by Streptozotocin (STZ), then the model of MIRI was established by ligating the reversible left anterior descending coronary artery for 30 min, and then reperfusing for 120 min. totally 40 male SD were randomly divided into five groups: the control group (NS), the ischemia reperfusion group (NIR), the diabetes control group (MS), the diabetic ischemia reperfusion group (MIR) and the diabetic ischemia reperfusion with icariin group (MIRI). The changes in blood glucose, body weight and living status were observed; the enzyme activity of serum CK-MB, LDH, GSH-Px and myocardium SOD and the content MDA and NO in myocardium were detected; the myocardial pathological changes were observed by HE staining; the myocardial Caspase-3, the Bcl-2, Bax protein expressions were detected by Western blot. The result showed that the diabetes model was successfully replicated; myocardial ischemia-reperfusion injury was more serious in diabetes rats; icariin can increase NO, SOD, GSH-Px, Bcl-2 protein expression, decrease MDA formation, CK-MB and LDH activities and Caspase-3 and Bcl-2 protein expressions and myocardial damage. The result suggested that icariin may play a protective role against ischemia reperfusion myocardial injury in diabetes rats by resisting oxidative stress and inhibiting cell apoptosis.
研究淫羊藿苷对糖尿病大鼠心肌缺血再灌注损伤（MIRI）模型的治疗作用及可能机制。采用链脲佐菌素（STZ）诱导糖尿病大鼠模型，然后通过结扎可逆性左冠状动脉前降支30分钟，再灌注120分钟建立MIRI模型。将40只雄性SD大鼠随机分为五组：对照组（NS）、缺血再灌注组（NIR）、糖尿病对照组（MS）、糖尿病缺血再灌注组（MIR）和糖尿病缺血再灌注淫羊藿苷组（MIRI）。观察血糖、体重和生存状态的变化；检测血清CK-MB、LDH、GSH-Px酶活性及心肌SOD活性和心肌组织中MDA、NO含量；通过HE染色观察心肌病理变化；采用Western blot检测心肌Caspase-3、Bcl-2、Bax蛋白表达。结果显示糖尿病模型成功复制；糖尿病大鼠心肌缺血再灌注损伤更严重；淫羊藿苷可增加NO、SOD、GSH-Px、Bcl-2蛋白表达，减少MDA生成、CK-MB和LDH活性以及Caspase-3和Bax蛋白表达并减轻心肌损伤。结果提示淫羊藿苷可能通过抗氧化应激和抑制细胞凋亡对糖尿病大鼠缺血再灌注心肌损伤发挥保护作用。
