Hong J L, Zhou H L, Bian R L
Zhongguo Yao Li Xue Bao. 1989 Nov;10(6):533-7.
Neutrophils (NP) cytoplasmic free Ca2+ concentrations ([Ca2+]i) under several conditions were measured with Quin 2 in rats. The process of loading Quin 2-AM into the cells followed by its hydrolysis was assured by monitoring the shift of fluorescent emission spectrum of Quin 2-AM to that of Quin 2. The resting [Ca2+]i of NP in Ca2(+)-containing and Ca2(+)-free solutions were 186 +/- 45 and 46 +/- 16 nmol/L, respectively, indicating extracellular calcium concentration ([Ca2+]0) plays an important role on [Ca2+]i. Among agents tested, prostaglandin E2 (PGE2) 100 nmol/L did not change [Ca2+]i significantly. Calcimycin 25 mumol/L, leukotriene B4(LTB4) 60 nmol/L and platelet activating factor (PAF) 10 nmol/L increased [Ca2+]i of NP in Ca2(+)-containing solution from 185 +/- 54, 175 +/- 36 and 188 +/- 54 to 814 +/- 67, 577 +/- 229 and 540 +/- 174 nmol/L, respectively. But, compound 48/80 3.2 micrograms/ml, LTB4 300 nmol/L, PAF 10 nmol/L and PAF 5 nmol/L plus LTB4 150 nmol/L did not change significantly [Ca2+]i of NP in Ca2(+)-free solution, indicating that these agonists can not release intracellularly stored Ca2+ or no stored Ca2+ in NP is available. The rises of [Ca2+]i produced by calcimycin, PAF and LTB4 were markedly inhibited by tetrandrine (Tet) 65 mumol/L. These results, as a whole, show that Tet inhibits the rises of [Ca2+]i of NP induced by calcimycin, PAF and LTB4 via decreasing Ca2+ influx. The Ca2+ antagonism in this case may be related to its anti-allergic actions.
用喹啉2在大鼠中测量了几种条件下中性粒细胞（NP）的细胞质游离钙离子浓度（[Ca2+]i）。通过监测喹啉2-AM荧光发射光谱向喹啉2的转变，确保了喹啉2-AM加载到细胞中并随后水解的过程。在含Ca2+和无Ca2+溶液中，NP的静息[Ca2+]i分别为186±45和46±16 nmol/L，表明细胞外钙离子浓度（[Ca2+]0）对[Ca2+]i起重要作用。在所测试的试剂中，100 nmol/L的前列腺素E2（PGE2）对[Ca2+]i没有显著影响。25 μmol/L的离子霉素、60 nmol/L的白三烯B4（LTB4）和10 nmol/L的血小板活化因子（PAF）使含Ca2+溶液中NP的[Ca2+]i分别从185±54、175±36和188±54升高到814±67、577±229和540±174 nmol/L。但是，3.2 μg/ml的化合物48/80、300 nmol/L的LTB4、10 nmol/L的PAF以及5 nmol/L的PAF加150 nmol/L的LTB4对无Ca2+溶液中NP的[Ca2+]i没有显著影响，表明这些激动剂不能释放细胞内储存的Ca2+或NP中没有可利用的储存Ca2+。65 μmol/L的粉防己碱（Tet）显著抑制了离子霉素、PAF和LTB4引起的[Ca2+]i升高。总体而言，这些结果表明Tet通过减少Ca2+内流来抑制离子霉素、PAF和LTB4诱导的NP的[Ca2+]i升高。这种情况下的Ca2+拮抗作用可能与其抗过敏作用有关。
