粉防己碱对兔血小板聚集及血小板活化因子生成的影响。
Effects of tetrandrine on rabbit platelet aggregation and platelet activating factor generation.
作者信息
Zhang M, Zhang L Z, Lu J S
机构信息
Department of Pharmacology, Third Military Medical College, Chongqing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 May;16(3):209-12.
AIM
To study the effects of tetrandrine (Tet) on platelet aggregation and platelet activating factor (PAF) generation in rabbit platelet-rich plasma (PRP).
METHODS
The aggregation rate of platelets induced by calcimycin (Cal) and PAF and the inhibition rate of Tet on platelet aggregation were measured. The amount of PAF in PRP stimulated with Cal and treated with Tet was also measured.
RESULTS
Cal 1-8 mumol.L(-1) and PAF 9.5-190.5 pmol.L(-1) induced platelet aggregation. At the final concentrations of 4-64 mumol.L(-1), Tet inhibited the aggregation induced by Cal 4 mumol.L(-1) and PAF 142.9 pmol.L-1. The IC50 (95% confidence limits) were 8.6 (6.0-12.2) mumol.L-1 for Cal and 14.0 (6.4-30.4) mumol.L(-1) for PAF. In the PRP aggregation by Cal, there was a marked increase in PAF content. Tet dependented the release of PAF from platelets by Cal in a concentration-dependent manner, with IC50 of 21 (8-54) mumol.L(-1).
CONCLUSION
The inhibition effect of Tet on platelet aggregation might be concerned with the reduction of endogenous PAF generation.
目的
研究粉防己碱（Tet）对兔富血小板血浆（PRP）中血小板聚集及血小板活化因子（PAF）生成的影响。
方法
测定离子霉素（Cal）和PAF诱导的血小板聚集率以及Tet对血小板聚集的抑制率。同时测定Cal刺激并用Tet处理的PRP中PAF的含量。
结果
Cal 1 - 8 μmol·L⁻¹和PAF 9.5 - 190.5 pmol·L⁻¹可诱导血小板聚集。在终浓度为4 - 64 μmol·L⁻¹时，Tet抑制4 μmol·L⁻¹ Cal和142.9 pmol·L⁻¹ PAF诱导的聚集。Cal的IC50（95%置信限）为8.6（6.0 - 12.2）μmol·L⁻¹，PAF的IC50为14.0（6.4 - 30.4）μmol·L⁻¹。在Cal诱导的PRP聚集中，PAF含量显著增加。Tet以浓度依赖方式抑制Cal诱导的血小板PAF释放，IC50为21（8 - 54）μmol·L⁻¹。
结论
Tet对血小板聚集的抑制作用可能与内源性PAF生成减少有关。
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