Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
Ultrason Sonochem. 2015 Sep;26:48-55. doi: 10.1016/j.ultsonch.2015.01.011. Epub 2015 Jan 17.
The effect of high intensity ultrasound (HIUS) may produce structural modifications on proteins through a friendly environmental process. Thus, it can be possible to obtain aggregates with a determined particle size, and altering a defined functional property at the same time. The objective of this work was to explore the impact of HIUS on the functionality of a denatured soy protein isolate (SPI) on foaming and interfacial properties. SPI solutions at pH 6.9 were treated with HIUS for 20 min, in an ultrasonic processor at room temperature, at 75, 80 and 85°C. The operating conditions were: 20 kHz, 4.27 ± 0.71 W and 20% of amplitude. It was determined the size of the protein particles, before and after the HIUS treatment, by dynamic light scattering. It was also analyzed the interfacial behavior of the different systems as well as their foaming properties, by applying the whipping method. The HIUS treatment and HIUS with temperature improved the foaming capacity by alteration of particle size whereas stability was not modified significantly. The temperature of HIUS treatment (80 and 85°C) showed a synergistic effect on foaming capacity. It was found that the reduction of particle size was related to the increase of foaming capacity of SPI. On the other hand, the invariable elasticity of the interfacial films could explain the stability of foams over time.
高强度超声（HIUS）的作用可能通过友好的环境过程对蛋白质产生结构修饰。因此，可以获得具有确定粒径的聚集体，同时改变定义明确的功能特性。本工作的目的是探索 HIUS 对变性大豆分离蛋白（SPI）起泡和界面性能的功能的影响。在室温下，将 pH 6.9 的 SPI 溶液在 75、80 和 85°C 的超声处理器中用 HIUS 处理 20 分钟。操作条件为：20 kHz、4.27±0.71 W 和 20%的振幅。通过动态光散射法测定 HIUS 处理前后蛋白质颗粒的粒径。还通过应用搅打法分析了不同体系的界面行为及其起泡性能。HIUS 处理和 HIUS 与温度联合处理通过改变粒径提高了起泡能力，而稳定性没有显著改变。HIUS 处理温度（80 和 85°C）对起泡能力表现出协同效应。研究发现，粒径的减小与 SPI 起泡能力的增加有关。另一方面，界面膜的不变弹性可以解释泡沫随时间的稳定性。
