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通过超声辅助交联肌原纤维蛋白乳液改善含乳液凝胶的流变学和摩擦学性能:对口腔加工模拟的洞察

Improving the rheological and tribological properties of emulsion-filled gel by ultrasound-assisted cross-linked myofibrillar protein emulsion: Insight into the simulation of oral processing.

作者信息

Tao Ye, Cai Jiaming, Wang Peng, Zhou Lei, Chai Jiale, Wang Zixu, Xu Xinglian

机构信息

State Key Laboratory of Meat Quality Control and Cultured Meat Development, Key Laboratory of Meat Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

State Key Laboratory of Meat Quality Control and Cultured Meat Development, Key Laboratory of Meat Processing, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Ultrason Sonochem. 2025 Jan;112:107205. doi: 10.1016/j.ultsonch.2024.107205. Epub 2024 Dec 16.

Abstract

This study aimed to investigate the effect of ultrasound-assisted cross-linking of myofibrillar protein (MP) emulsions on the enhancement of rheological and tribological properties of emulsion-filled gel. The micro-morphology, texture, water hold capacity (WHC), chemical forces, linear shear rheological behavior, large amplitude oscillatory shear (LAOS), oil-released content, and simulated oral friction of the water-filled gel (WP-G), the original MP fabricated emulsion-filled gel (NP-G), the crosslinked MP fabricated emulsion-filled gel (NPG-G), and the ultrasound treated crosslinked MP fabricated emulsion-filled gel (NPGU-G) were determined. Results indicated that emulsion as filler phase significantly improved the rheological and tribological properties of the gel, especially for the ultrasound-assisted MP emulsion-filled gel (NPGU-G) group, the smaller droplet size of emulsion contributed to the density and structural uniformity of the gel. Based on the excellent hydrophobic interaction between emulsion droplets and protein matrix, the NPGU-G group presented enhanced hardness, gumminess, chewiness, hydrophobic interaction, creep-recovery behavior, and the retarded transition of nonlinear response. Furthermore, the lower oil-released content and reduced friction coefficient in the NPGU-G group also indicated that the smaller emulsion droplets contributed to the gel quality and mouth lubrication. Consequently, this study demonstrated that ultrasound-assisted cross-linked MP emulsion with smaller droplets can be successfully filled into gel structures, form a denser network structure, and improve the quality of the emulsion-filled gel.

摘要

本研究旨在探究肌原纤维蛋白(MP)乳液的超声辅助交联对增强乳液填充凝胶的流变学和摩擦学性能的影响。测定了水填充凝胶(WP-G)、原始MP制备的乳液填充凝胶(NP-G)、交联MP制备的乳液填充凝胶(NPG-G)以及超声处理的交联MP制备的乳液填充凝胶(NPGU-G)的微观形态、质地、持水能力(WHC)、化学力、线性剪切流变行为、大振幅振荡剪切(LAOS)、油释放量和模拟口腔摩擦力。结果表明,乳液作为填充相显著改善了凝胶的流变学和摩擦学性能,特别是对于超声辅助MP乳液填充凝胶(NPGU-G)组,乳液较小的液滴尺寸有助于凝胶的密度和结构均匀性。基于乳液液滴与蛋白质基质之间优异的疏水相互作用,NPGU-G组表现出增强的硬度、黏性、咀嚼性、疏水相互作用、蠕变-回复行为以及非线性响应的延迟转变。此外,NPGU-G组较低的油释放量和降低的摩擦系数也表明较小的乳液液滴有助于凝胶质量和口腔润滑。因此,本研究表明,具有较小液滴的超声辅助交联MP乳液能够成功填充到凝胶结构中,形成更致密的网络结构,并提高乳液填充凝胶的质量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e074/11718346/505c9af2f27e/ga1.jpg

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