Tvede N, Heilmann C, Halkjaer-Kristensen J, Pedersen B K
Laboratory of Medical Immunology, Rigshospitalet, Copenhagen, Denmark.
J Clin Lab Immunol. 1989 Dec;30(4):169-73.
Blood mononuclear cells from 20 healthy untrained volunteers were isolated before, during, 2 hr and 24 hr after bicycle exercise at 80% of VO2 max for 1 hr. A reverse plaque forming cell assay was used to investigate B-lymphocyte function. Stimulation with pokeweed mitogen, recombinant interleukin 2 and Epstein-Barr virus resulted in significant decreases in numbers of IgG-, IgM- and IgA-secreting blood cells during as well as 2 hr after exercise, with reversal to pre-exercise values 24 hr later. During and after physical activity we found an unchanged concentration of CD20-positive B lymphocytes suggesting that the suppression of immunoglobulin secreting cells was not due to changes in numbers of B lymphocytes. A decline in CD4/CD8 ratio was measured only during exercise with normalization after exercise. Therefore the B-lymphocyte suppression, most pronounced 2 hr after exercise, was presumably not due to changes in T lymphocytes also indicated in the experiments using EBV-stimulated cultures since EBV acts directly on B lymphocytes. 2 hr after physical activity an increased level of CD14-positive monocytes were observed and the monocytes expressed higher levels of surface HLA-DR during as well as 2 hr after exercise. Addition of indomethacin caused an increased response only in the IL-2 stimulated cultures, suggesting that IL-2-sensitive activated B lymphocytes are downregulated by prostaglandins. Purified B lymphocytes produced plaques only after EBV-stimulation, and in these cultures no exercise-induced suppression was found, likewise suggesting an inhibitory effect of the activated monocytes.
从20名未经训练的健康志愿者中分离出血液单核细胞，分别在以最大摄氧量（VO2 max）的80%进行1小时自行车运动前、运动期间、运动后2小时和24小时进行。采用反向空斑形成细胞试验来研究B淋巴细胞功能。用美洲商陆丝裂原、重组白细胞介素2和爱泼斯坦-巴尔病毒刺激后，运动期间以及运动后2小时，分泌IgG、IgM和IgA的血细胞数量显著减少，24小时后恢复到运动前水平。在体力活动期间及之后，我们发现CD20阳性B淋巴细胞的浓度没有变化，这表明免疫球蛋白分泌细胞的抑制并非由于B淋巴细胞数量的改变。仅在运动期间检测到CD4/CD8比值下降，运动后恢复正常。因此，运动后2小时最为明显的B淋巴细胞抑制，可能不是由于T淋巴细胞的变化，因为在使用爱泼斯坦-巴尔病毒刺激培养物的实验中也表明，爱泼斯坦-巴尔病毒直接作用于B淋巴细胞。体力活动2小时后，观察到CD14阳性单核细胞水平升高，并且在运动期间以及运动后2小时，单核细胞表面HLA-DR的表达水平更高。添加吲哚美辛仅在白细胞介素2刺激的培养物中引起反应增加，这表明白细胞介素2敏感的活化B淋巴细胞被前列腺素下调。纯化的B淋巴细胞仅在爱泼斯坦-巴尔病毒刺激后产生空斑，并且在这些培养物中未发现运动诱导的抑制作用，同样表明活化的单核细胞具有抑制作用。
